CD7 Human, Sf9
Sf9, Baculovirus cells.
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD7 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 164 amino acids (26-180a.a.) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). CD7 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Sf9, Baculovirus cells.
ADLAQEVQQS PHCTTVPVGA SVNITCSTSG GLRGIYLRQL GPQPQDIIYY EDGVVPTTDR RFRGRIDFSG SQDNLTITMH RLQLSDTGTY TCQAITEVNV YGSGTLVLVT EEQSQGWHRC SDAPPRASAL PAPPTGSALP DPQTASALPD PPAASALPHH HHHH.
Product Science Overview
CD7, also known as T-cell antigen CD7, GP40, LEU-9, Tp40, and TP41, is a type I transmembrane glycoprotein. It is primarily expressed on pluripotential hematopoietic cells, nearly all human thymocytes, and some peripheral blood T cells. The CD7 molecule is notably absent in a subset of human T cells under certain physiological conditions. An increase in CD7-negative T cells can be observed in various inflammatory skin diseases .
The CD7 protein is a single, glycosylated polypeptide chain consisting of 164 amino acids (26-180a.a.) and has a molecular mass of approximately 17.5 kDa. When analyzed using SDS-PAGE, the molecular size appears to be between 18-28 kDa . The recombinant CD7 protein produced in Sf9 Baculovirus cells includes a 6-amino acid His tag at the C-terminus, which facilitates its purification through proprietary chromatographic techniques .
The recombinant CD7 protein is produced in Sf9 Baculovirus cells. Sf9 cells are derived from the fall armyworm (Spodoptera frugiperda) and are commonly used in the baculovirus expression system for producing recombinant proteins. The CD7 protein is expressed as a glycosylated polypeptide chain and is purified to a purity greater than 95.0% as determined by SDS-PAGE .
For short-term storage (2-4 weeks), the CD7 protein should be kept at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To ensure stability during long-term storage, adding a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) is advisable. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
The recombinant CD7 protein is used in various laboratory research applications. It is important to note that ProSpec’s products, including the CD7 protein, are intended for laboratory research use only and are not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .
