CD7 Antibody

CD7 Antibody, Mouse Anti Human
Cat. No.
BT14294
Source
Synonyms
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
CD7, also known as T-cell antigen CD7, is a type I transmembrane glycoprotein present on various cells, including pluripotent hematopoietic cells, almost all human thymocytes, and a subset of peripheral blood T cells. Notably, a subset of human T cells lacks the CD7 molecule under certain physiological conditions. An increase in CD7-negative T cells is observed in various inflammatory skin diseases.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer containing PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store the antibody at 4°C. For long-term storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1000 is recommended.
Synonyms
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Purification Method
CD7 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1B9AT.
Immunogen
Anti-human CD7 mAb, clone PAT1B9AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD7 protein 26-180 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

The CD7 antibody, specifically the mouse anti-human variant, is a monoclonal antibody that targets the CD7 antigen. CD7 is a cell surface glycoprotein that plays a crucial role in the immune system. This antibody is widely used in various scientific applications, including flow cytometry, immunohistochemistry, western blotting, immunocytochemistry, and enzyme-linked immunosorbent assay (ELISA).

CD7 Antigen

CD7, also known as TP41 or GP40, is a 40-kilodalton (kDa) type I transmembrane glycoprotein. It is a member of the immunoglobulin superfamily (IgSF) and is expressed on the surface of T cells, natural killer (NK) cells, and thymocytes . CD7 is also found on tumor cells in certain cases of acute myeloid leukemia .

Production of CD7 Antibody

The mouse anti-human CD7 antibody is produced by immunizing mice with human peripheral blood lymphocytes. The spleen cells from these immunized mice are then fused with myeloma cells to create hybridoma cells that produce the monoclonal antibody . The antibody is purified using affinity chromatography on Protein A from ascites fluid.

Applications

The CD7 antibody is utilized in various research and diagnostic applications:

  • Flow Cytometry: Used to analyze the expression of CD7 on the surface of cells.
  • Immunohistochemistry: Helps in the detection of CD7 in tissue samples.
  • Western Blotting: Used to identify CD7 protein in cell lysates.
  • Immunocytochemistry: Allows for the visualization of CD7 in cultured cells.
  • ELISA: Used to quantify the presence of CD7 in various samples .
Specificity and Validation

The CD7 antibody is highly specific to the CD7 antigen and has been validated in multiple experiments. It has been tested on human, mouse, and rat samples to confirm its specificity . The antibody is available in various conjugated forms, including FITC, PE, APC, and biotin, to suit different experimental needs .

Storage and Handling

The CD7 antibody is typically shipped at ambient temperature and should be stored at -20°C upon receipt. For short-term use, aliquots can be kept at 2-8°C for up to four weeks. It is important to avoid repeated freezing and thawing to prevent denaturation of the antibody .

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