CD7 Human
CD7 Human Recombinant
Cat. No.
BT30510
Source
E.coli.
Synonyms
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (26-180 a.a) and having a molecular mass of 18.8 kDa.
CD7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CD7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD7, also known as T-cell antigen CD7, is a transmembrane glycoprotein classified as a type I molecule. It is present on cells involved in hematopoietic potential, the majority of thymocytes in humans, and a subset of T cells circulating in peripheral blood. Under specific physiological circumstances, a fraction of human T cells lack the CD7 molecule. An increase in the population of CD7-negative T cells is observed in various inflammatory skin disorders.
Description
Recombinant human CD7, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It consists of 178 amino acids, spanning from position 26 to 180, resulting in a molecular weight of 18.8 kDa. A 23-amino acid His-tag is fused to the N-terminus of the CD7 protein. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The CD7 protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For optimal storage, keep the CD7 protein at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. To maintain protein integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of the CD7 protein is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
T-cell antigen CD7, GP40, LEU-9, Tp40, TP41, CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSAQEVQQS PHCTTVPVGA SVNITCSTSG GLRGIYLRQL GPQPQDIIYY EDGVVPTTDR RFRGRIDFSG SQDNLTITMH RLQLSDTGTY TCQAITEVNV YGSGTLVLVT EEQSQGWHRC SDAPPRASAL PAPPTGSALP DPQTASALPD PPAASALP.
Product Science Overview
Structure and Function
The mature human CD7 protein consists of:
- A 155 amino acid extracellular domain (ECD)
- A 21 amino acid transmembrane segment
- A 39 amino acid cytoplasmic domain
Within the extracellular domain, human CD7 shares 43% and 41% amino acid sequence identity with mouse and rat CD7, respectively . The protein plays a crucial role in T-cell interactions and signaling, contributing to the immune response.
Recombinant Human CD7
Recombinant human CD7 is produced using human embryonic kidney (HEK293) cells . The recombinant protein typically includes a polyhistidine tag at the C-terminus for purification purposes . The secreted recombinant human CD7 consists of 166 amino acids and has a predicted molecular mass of 18 kilodaltons . However, due to glycosylation, it migrates with an apparent molecular mass of 30-35 kilodaltons in SDS-PAGE under reducing conditions .
Applications
Stability and Storage
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