CD4 Antibody

CD4 is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It plays a crucial role in the immune system by aiding in the activation and development of T cells. The “Mouse Anti-Human CD4” antibody is a monoclonal antibody derived from mice that specifically targets the human CD4 protein. This antibody is widely used in research and clinical settings for various applications, including flow cytometry, immunohistochemistry, and immunofluorescence.

CD4 is composed of four extracellular immunoglobulin-like domains (D1-D4), a transmembrane region, and a short cytoplasmic tail. The primary function of CD4 is to act as a co-receptor for the T cell receptor (TCR) during the recognition of antigens presented by Major Histocompatibility Complex (MHC) class II molecules. The interaction between CD4 and the MHC class II molecule enhances the sensitivity and specificity of the TCR-antigen interaction, which is essential for T cell activation and the subsequent immune response .

The Mouse Anti-Human CD4 antibody is a monoclonal antibody produced by immunizing mice with human CD4 protein. This antibody binds specifically to the D1 domain of the CD4 molecule, which is involved in the interaction with MHC class II molecules. The antibody is typically purified from tissue culture supernatant or ascites using affinity chromatography, ensuring high purity and specificity .

1. Flow Cytometry: The Mouse Anti-Human CD4 antibody is commonly used in flow cytometry to identify and quantify CD4+ T cells in various samples. This application is crucial for monitoring immune responses, diagnosing immunodeficiencies, and evaluating the efficacy of immunotherapies .
2. Immunohistochemistry: This antibody is also used in immunohistochemistry to detect CD4 expression in tissue sections. This technique is valuable for studying the distribution and localization of CD4+ cells in different tissues and understanding their role in various diseases .
3. Immunofluorescence: In immunofluorescence assays, the Mouse Anti-Human CD4 antibody can be conjugated with fluorescent dyes to visualize CD4+ cells under a fluorescence microscope. This application is useful for studying cell morphology and interactions in various experimental settings .

The Mouse Anti-Human CD4 antibody should be stored at 4°C and protected from prolonged exposure to light. It should not be frozen, as this can affect its binding activity. The antibody is typically supplied in an aqueous buffered solution containing sodium azide as a preservative .