CD4 biotin Antibody
CD4-Biotinylated, Mouse Anti-Human
Cat. No.
BT29926
Source
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Introduction
CD4 is a cell surface glycoprotein present on mature helper T cells, immature thymocytes, monocytes, and macrophages. A subset of cytotoxic T cells also express CD4. Approximately 65% of circulating T cells are CD4+. Mature T cells express either CD4 or CD8, but not both. During development, T cells initially express both CD4 and CD8 but undergo differentiation in the thymus to become specialized.
Formulation
Supplied at a concentration of 1 mg/ml in PBS buffer after reconstitution.
Storage Procedures
Store the lyophilized antibody at 4°C. After reconstitution, the antibody can be stored at 4°C for up to one month. For long-term storage, aliquot the reconstituted antibody and store at -20°C.
Solubility
Reconstitute the antibody by adding deionized water (H2O). Gently mix the solution by inverting the vial several times. Rinse the sides of the vial with reconstituted antibody. Allow the reconstituted antibody to sit for 30-60 seconds at room temperature before use.
Applications
This antibody has applications in blocking and staining procedures. It effectively blocks the binding of HIV to CD4. For staining applications, it is recommended to use 10 µl of antibody per 10^6 cells. The optimal titer for blocking T cell activation should be determined empirically by the investigator.
Available Conjugates
This antibody is also offered conjugated to FITC.
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Clone
hCD4.
Immunogen
Purified human PBL CD4+ T cells.
Ig Subclass
Mouse IgG2b.
Product Science Overview
Function and Importance
Biotinylation and Its Benefits
Biotinylation is the process of attaching biotin to proteins and other macromolecules. The biotinylated form of CD4, specifically the mouse anti-human CD4 antibody, is used in various research and diagnostic applications. Biotinylation enhances the detection and purification of proteins due to the strong affinity between biotin and streptavidin .
Applications
The biotinylated mouse anti-human CD4 antibody is widely used in flow cytometry, immunohistochemistry, and other immunoassays. It is particularly useful in studying T cell activation, HIV research, and immune response mechanisms . The antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding, inhibiting syncytia formation .
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