sCD4 Antibody

The CD4 molecule, also known as Cluster of Differentiation 4, is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It plays a crucial role in the immune response by assisting in the activation of T cells. CD4 acts as a co-receptor that enhances the interaction between the T cell receptor (TCR) and the major histocompatibility complex (MHC) class II molecules on antigen-presenting cells.

Rat Anti-Mouse CD4 antibodies are specifically designed to target and bind to the CD4 molecule on mouse cells. These antibodies are produced by immunizing rats with mouse CD4 protein, leading to the generation of an immune response in the rat. The resulting antibodies are then harvested and purified for use in various applications.

Rat Anti-Mouse CD4 antibodies are used in a variety of scientific applications, including:

• Flow Cytometry: To identify and quantify CD4+ T cells in a sample.
• Immunohistochemistry (IHC): To visualize CD4+ cells in tissue sections.
• Western Blotting: To detect CD4 protein in cell lysates.
• Immunoprecipitation: To isolate CD4 protein from a mixture of proteins.

Several clones of Rat Anti-Mouse CD4 antibodies are available, each with specific characteristics and applications. Some commonly used clones include:

• GK1.5: This clone is widely used in flow cytometry and immunohistochemistry .
• RM4-5: This clone is used in flow cytometry and has been validated for its specificity and performance .

Rat Anti-Mouse CD4 antibodies are essential tools in immunological research. They enable scientists to study the role of CD4+ T cells in various immune responses, including infection, autoimmunity, and cancer. By understanding the function and regulation of CD4+ T cells, researchers can develop new therapeutic strategies for treating immune-related diseases.