CD4 Antibody, Biotin
Description
Product Specs
Product Science Overview
Rat Anti-Mouse CD4, Biotinylated is a monoclonal antibody used extensively in immunological research. This antibody is specifically designed to target and bind to the CD4 antigen present on the surface of mouse T cells. The biotinylation of the antibody allows for easy detection and quantification using various biotin-streptavidin based assays.
CD4 is a type I membrane glycoprotein that belongs to the immunoglobulin superfamily. It is predominantly expressed on thymocytes and a subset of mature T lymphocytes. CD4 functions as a co-receptor that assists the T cell receptor (TCR) in recognizing antigens presented by class II major histocompatibility complexes (MHC II) on antigen-presenting cells . Additionally, CD4 plays a crucial role in the immune response by aiding in the activation and differentiation of T helper cells.
The Rat Anti-Mouse CD4 antibody is produced using hybridoma technology. This involves the fusion of rat myeloma cells with spleen cells from a rat immunized with mouse thymocytes. The resulting hybridoma cells are screened for the production of antibodies that specifically bind to the CD4 antigen. Once identified, these hybridoma cells are cultured to produce large quantities of the monoclonal antibody .
Biotinylation is a process where biotin molecules are covalently attached to the antibody. This modification allows the antibody to be easily detected and quantified using biotin-streptavidin based assays. Streptavidin, a protein with a high affinity for biotin, can be conjugated to various reporter molecules such as enzymes, fluorophores, or beads. When the biotinylated antibody binds to its target antigen, the streptavidin-conjugated reporter can be used to visualize or measure the antigen-antibody interaction .
Rat Anti-Mouse CD4, Biotinylated is widely used in various immunological assays, including:
- Flow Cytometry: This technique allows for the analysis of cell surface markers on individual cells within a mixed population. The biotinylated antibody can be detected using streptavidin-conjugated fluorophores, enabling the identification and quantification of CD4+ T cells .
- Immunohistochemistry: This method involves the staining of tissue sections to visualize the distribution and localization of specific antigens. The biotinylated antibody can be used in combination with streptavidin-conjugated enzymes to produce a colorimetric or fluorescent signal .
- Immunoprecipitation: This technique is used to isolate and purify specific antigens from a complex mixture. The biotinylated antibody can be used to capture CD4+ T cells, which can then be isolated using streptavidin-coated beads .
