CD3 Anti-Human

Mouse Anti-Human CD3 antibodies are monoclonal antibodies developed in mice that specifically target the human CD3 complex. These antibodies are widely used in various scientific applications, including:

• Flow Cytometry: For identifying and quantifying T cells in a sample.
• Immunohistochemistry (IHC): For detecting CD3 in tissue sections, aiding in the classification of T-cell neoplasms .
• Western Blot: For analyzing protein expression levels.
• Immunocytochemistry: For visualizing CD3 in cell cultures.
• Immunoprecipitation: For isolating CD3 proteins from a mixture.

The OKT3 clone is a well-known monoclonal antibody that reacts with an epitope on the epsilon-subunit within the human CD3 complex. It is capable of inducing T cell activation and is used in various functional assays . The antibody is typically purified by affinity chromatography and is available in different formulations, including phosphate-buffered solutions with sodium azide for preservation .

The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. It is important not to freeze the solution to maintain its efficacy .

Mouse Anti-Human CD3 antibodies are essential tools in immunology research. They help in understanding T cell behavior, immune responses, and the mechanisms underlying various diseases. These antibodies also play a crucial role in the development of immunotherapies and diagnostic techniques.