CD3 Antibody, Biotin

CD3, Mouse Anti-Human, Biotin is a monoclonal antibody that targets the CD3 complex on human T cells. This antibody is conjugated with biotin, a vitamin that allows for easy detection and purification using avidin or streptavidin-based systems. The CD3 complex is a critical component of the T-cell receptor (TCR) complex, which plays a vital role in the immune response.

The CD3 complex is composed of several subunits: CD3ε, CD3γ, CD3δ, and CD3ζ (CD247). These subunits associate with the TCR heterodimer, which can be either α/β or γ/δ. The CD3 complex is essential for the proper assembly, trafficking, and surface expression of the TCR. It is found on all mature T cells, natural killer T (NKT) cells, and some thymocytes .

The primary function of the CD3 complex is to transduce signals from the TCR upon antigen recognition. This signal transduction is crucial for T cell activation, proliferation, and differentiation. The CD3 complex plays a central role in the immune response, enabling T cells to recognize and respond to pathogens .

The mouse anti-human CD3 antibody is a monoclonal antibody that specifically binds to the CD3ε subunit of the CD3 complex. This antibody is produced by immunizing mice with human CD3ε and then isolating the specific antibody-producing cells. The antibody is then purified using affinity chromatography .

Biotin is a small molecule that can be easily conjugated to antibodies. The biotinylation of the mouse anti-human CD3 antibody allows for its detection and purification using avidin or streptavidin-based systems. Avidin and streptavidin have a high affinity for biotin, making this conjugation method highly effective for various applications .

The CD3, Mouse Anti-Human, Biotin antibody is used in a variety of research and clinical applications, including:

• Flow Cytometry: Used to identify and quantify T cells in a sample.
• Immunohistochemistry: Used to detect CD3-expressing cells in tissue sections.
• Western Blotting: Used to detect CD3 proteins in cell lysates.
• T Cell Activation: Used to stimulate T cells in vitro for functional studies .

The antibody solution should be stored undiluted between 2°C and 8°C. It should not be frozen, as this can affect its stability and functionality. The recommended usage for flow cytometric staining is ≤0.5 µg per million cells in a 100 µL volume .