CD3 FITC Antibody

CD3, Mouse Anti-Human FITC is a monoclonal antibody used extensively in immunology research, particularly in the study of T cells. This antibody is conjugated with fluorescein isothiocyanate (FITC), a fluorescent dye, which allows for the detection and analysis of CD3 molecules on the surface of human T cells using flow cytometry.

The CD3 molecule is a complex of proteins that is crucial for T cell activation and signal transduction. It is composed of several subunits, including CD3ε, CD3γ, CD3δ, and CD3ζ (CD247), along with the T-cell receptor (TCR) heterodimer (α/β or γ/δ). The CD3 complex is found on all mature T cells, natural killer (NK) T cells, and some thymocytes .

The Mouse Anti-Human CD3 antibody specifically binds to the human CD3ε-chain, a 20 kDa subunit of the CD3/TCR complex. This antibody is typically of the IgG1 isotype and is produced by immunizing mice with human CD3 antigens. The antibody is then purified using affinity chromatography .

Fluorescein isothiocyanate (FITC) is a widely used fluorescent dye for labeling antibodies. The conjugation of FITC to the Mouse Anti-Human CD3 antibody allows for the visualization of CD3 molecules on T cells through flow cytometry. FITC emits a green fluorescence when excited by a specific wavelength of light, making it an ideal marker for detecting and quantifying CD3 expression .

The primary application of CD3, Mouse Anti-Human FITC is in flow cytometry, a technique used to analyze the physical and chemical characteristics of cells. By staining human peripheral blood lymphocytes with this antibody, researchers can assess the expression of CD3 on T cells, which is essential for studying T cell development, activation, and function .

The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. It is important to avoid freezing the antibody, as this can lead to a loss of activity. Additionally, the antibody contains sodium azide as a preservative, which requires careful handling and disposal .

When working with CD3, Mouse Anti-Human FITC, it is essential to follow appropriate safety practices, including the use of personal protective equipment. Sodium azide, a component of the antibody solution, can be hazardous if not handled properly. It is crucial to follow federal, state, and local regulations for the disposal of this reagent .