CD28 Human
Sf9, Baculovirus cells.
T-cell-specific surface glycoprotein CD28, TP44, CD_antigen: CD28, T-cell-specific surface glycoprotein CD28 isoform 1.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD28 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 376 amino acids (19-152a.a.) and having a molecular mass of 42.4kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). CD28 is expressed with a 242 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
T-cell-specific surface glycoprotein CD28, TP44, CD_antigen: CD28, T-cell-specific surface glycoprotein CD28 isoform 1.
Sf9, Baculovirus cells.
ADPNKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKPLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Product Science Overview
CD28 functions as a co-stimulatory receptor that is essential for the full activation of T cells. It works in conjunction with the T-cell receptor (TCR) to amplify TCR signals and deliver unique signals that control intracellular biochemical events, ultimately altering the gene expression program of T cells . This co-stimulatory pathway is vital for the regulation of T and B cell responses, and it involves interactions with its ligands, B7-1 (CD80) and B7-2 (CD86) .
Recombinant human CD28 is produced using advanced biotechnological methods. The DNA sequence encoding human CD28 is expressed in host cells, such as HEK293 cells, to produce the recombinant protein. The recombinant human CD28 protein is often biotinylated and verified through various methods, including HPLC and MALS . The recombinant protein is typically provided as a lyophilized powder and can be reconstituted for use in various research applications .
Recombinant human CD28 is widely used in immunological research to study T-cell activation and co-stimulation. It is also utilized in the development of immunotherapies and in the investigation of immune responses. The ability to produce recombinant CD28 allows researchers to explore its functions and interactions in a controlled environment, providing valuable insights into immune regulation and potential therapeutic targets.
