CD28 Antibody
Description
Product Specs
Product Science Overview
CD28 (Cluster of Differentiation 28) is a protein expressed on T cells that provides essential co-stimulatory signals required for T cell activation and survival . It is a type I transmembrane glycoprotein of the immunoglobulin superfamily and is constitutively expressed on the surface of 80% of CD4+ T cells and 50% of CD8+ T cells in humans, and 100% on both CD4+ and CD8+ T cells in mice . CD28 plays a crucial role in the immune response by enhancing the production of various interleukins, particularly IL-6, when T cells are stimulated through CD28 in conjunction with the T-cell receptor (TCR) .
CD28 is involved in several key processes:
- T-cell Activation: It provides the necessary second signal for T-cell activation, which is essential for the induction of cell proliferation and cytokine production .
- Cytokine Production: CD28 enhances the production of IL-4 and IL-10 in T cells in conjunction with TCR/CD3 ligation and CD40L costimulation .
- T-cell Survival: It promotes T-cell survival by enhancing the production of survival signals .
Hamster Anti-Mouse CD28 antibodies are monoclonal antibodies derived from hamsters and are specific to the CD28 molecule in mice. These antibodies are commonly used in research to study T-cell activation and function. One of the well-known clones is 37.51, which is used in various applications such as flow cytometry, immunohistochemistry, and in vitro costimulation .
- Flow Cytometry: Hamster Anti-Mouse CD28 antibodies are used to detect and quantify CD28 expression on T cells and NK cells in mouse models .
- Immunohistochemistry: These antibodies are used to stain tissue sections to study the localization and expression of CD28 in different tissues .
- Costimulation: The 37.51 antibody clone is known to augment T-cell proliferation and cytokine production, providing a costimulatory signal for CTL induction .
