CD28 Antibody, Biotin

The Hamster Anti-Mouse CD28 antibody is a monoclonal antibody derived from Syrian Hamster. It is specifically designed to target the CD28 antigen on mouse cells. This antibody is often biotinylated, which means it is conjugated with biotin, a vitamin that allows for easy detection and amplification in various assays .

• Clone: 37.51
• Isotype: Syrian Hamster IgG
• Immunogen: Mouse EL-4 (T-cell lymphoma) cells

The biotinylated Hamster Anti-Mouse CD28 antibody is widely used in immunological research for several applications:

1. Flow Cytometry: It is used to stain cell suspensions from peripheral lymphoid tissues to detect T lymphocytes and NK cells. The biotinylation allows for signal amplification using a secondary reagent such as PE Streptavidin .
2. Immunohistochemistry: It can be used to stain frozen tissue sections, providing insights into the localization and expression of CD28 in various tissues .
3. Costimulation: The antibody can augment proliferation and cytokine production by activated T and NK cells, providing a costimulatory signal for cytotoxic T lymphocyte (CTL) induction .
4. Blocking and Cytotoxicity: It can be used in blocking assays to inhibit specific interactions and in cytotoxicity assays to study immune cell functions .

The antibody is typically purified from tissue culture supernatant or ascites by affinity chromatography. It is provided in an aqueous buffered solution containing protein stabilizer and no preservatives. The recommended storage condition is at 4°C, and it should be handled under aseptic conditions to maintain its stability and functionality .