CD28 Antibody, FITC

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44, and is a member of the immunoglobulin superfamily. It is expressed on thymocytes, most peripheral T cells, and natural killer (NK) cells . CD28 plays a crucial role in the activation and proliferation of T and NK cells by acting as a costimulatory receptor. Its ligands include CD80 (B7-1) and CD86 (B7-2), which are expressed on antigen-presenting cells .

The Hamster Anti-Mouse CD28 antibody is a monoclonal antibody derived from Syrian or Armenian hamsters. This antibody specifically binds to the CD28 molecule on mouse cells. It is commonly used in various immunological assays, including flow cytometry, immunoprecipitation, and in vitro costimulation of T and NK cells .

FITC (Fluorescein Isothiocyanate) is a fluorescent dye that is commonly conjugated to antibodies for use in flow cytometry and other fluorescence-based assays. The FITC-conjugated Hamster Anti-Mouse CD28 antibody allows for the detection and analysis of CD28 expression on mouse cells using flow cytometry .

The Hamster Anti-Mouse CD28-FITC antibody is widely used in research to study T cell activation, proliferation, and cytokine production. It has been reported to augment in vitro T cell proliferation and cytokine production, and promote cytotoxic T lymphocyte (CTL) development . The antibody is also used in immunohistochemical staining of acetone-fixed frozen sections and in vitro blocking of allogeneic mixed leukocyte response .

The CD28 antibody solution should be stored undiluted between 2°C and 8°C. It is recommended to avoid exposure to light to preserve the integrity of the FITC conjugate .