CD21 Antibody, FITC
CD21, Mouse Anti-Human, FITC
Cat. No.
BT29092
Source
Synonyms
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21 antigen, CR2, C3DR, CD21, SLEB9.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CD21, primarily found on mature B cells and follicular dendritic cells, plays a crucial role in B cell development and activation. This receptor, weakly expressed on immature thymocytes and T lymphocytes, emerges post pre-B-cell stage and persists throughout peripheral B-cell development, disappearing upon terminal differentiation into plasma cells. In vitro B cell stimulation also gradually diminishes CD21 expression. Functionally, CD21 serves as a receptor for complement components C3d, C3dg, and iC3b, as well as for Epstein-Barr Virus (EBV) and Interferon alpha (IFNα). It interacts with CD23 and associates with CD19, CD81, and Leu13, forming a large signal-transduction complex essential for B cell activation.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) upon reconstitution.
Storage Procedures
Store the lyophilized antibody at 4°C. Following reconstitution, use within one month. For long-term storage, aliquot the reconstituted antibody and store at -20°C.
Solubility
Reconstitute the antibody by adding the recommended volume of deionized or distilled water (H2O) to the vial. Gently mix the solution, ensuring the sides of the vial are rinsed, and allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for staining procedures. A recommended concentration of 10 µl of antibody per 1,000,000 cells is suggested for optimal staining.
Available Conjugates
In addition to the FITC conjugate, this antibody is also available in unconjugated and biotinylated formats. For staining with either biotin-conjugated or FITC-conjugated antibodies, a concentration of 5-10 µl per 1,000,000 cells is recommended.
Synonyms
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21 antigen, CR2, C3DR, CD21, SLEB9.
Purification Method
Protein-A.
Type
Mouse Anti Human Monoclonal.
Clone
hCD21.
Immunogen
Purified human B-Cells.
Ig Subclass
Mouse IgG2a.
Product Science Overview
Introduction
CD21, also known as Complement Receptor 2 (CR2), is a 145 kDa glycosylated type I integral membrane protein. It plays a crucial role in the immune system by acting as a receptor for the C3d complement fragment and the Epstein-Barr virus (EBV) . The FITC Mouse Anti-Human CD21 antibody is a monoclonal antibody that specifically binds to CD21 and is conjugated with fluorescein isothiocyanate (FITC) for use in flow cytometry.
CD21 Expression
CD21 is expressed on various cell types, including:
- Mature B cells: CD21 is prominently found on the surface of mature B cells, where it plays a role in B-cell activation and proliferation.
- Follicular dendritic cells: These cells, found in lymphoid follicles, also express CD21.
- Epithelial cells: Some epithelial cells express CD21, although to a lesser extent.
- T cells and thymocytes: CD21 is weakly expressed on a subset of mature T cells and thymocytes .
Function of CD21
CD21 is involved in several critical functions within the immune system:
- B-cell activation: CD21 enhances B-cell receptor (BCR) signaling, leading to increased antibody production.
- Immune response modulation: CD21 may play a role in modulating T-cell function during immune responses to infections by lymphotropic viruses .
- Receptor for EBV: CD21 serves as a receptor for the Epstein-Barr virus, facilitating its entry into B cells .
FITC Mouse Anti-Human CD21 Antibody
The FITC Mouse Anti-Human CD21 antibody is a monoclonal antibody that binds specifically to CD21. It is conjugated with FITC, a fluorescent dye, making it suitable for use in flow cytometry. This antibody is commonly used in research to study CD21 expression on human peripheral blood lymphocytes .
Applications
Storage and Handling
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