CD21 Human
Sf9, Baculovirus cells.
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21 antigen, CR2, C3DR, CD21, SLEB9.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD21 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 959 amino acids (21-971aa) and having a molecular mass of 105.2kDa.
CD21 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21 antigen, CR2, C3DR, CD21, SLEB9.
Sf9, Baculovirus cells.
ISCGSPPPIL NGRISYYSTP IAVGTVIRYS CSGTFRLIGE KSLLCITKDK VDGTWDKPAP
KCEYFNKYSS CPEPIVPGGY KIRGSTPYRH GDSVTFACKT NFSMNGNKSV WCQANNMWGP
TRLPTCVSVF PLECPALPMI HNGHHTSENV GSIAPGLSVT YSCESGYLLV GEKIINCLSS
GKWSAVPPTC EEARCKSLGR FPNGKVKEPP ILRVGVTANF FCDEGYRLQG PPSSRCVIAG
QGVAWTKMPV CEEIFCPSPP PILNGRHIGN SLANVSYGSI VTYTCDPDPE EGVNFILIGE
STLRCTVDSQ KTGTWSGPAP RCELSTSAVQ CPHPQILRGR MVSGQKDRYT YNDTVIFACM
FGFTLKGSKQ IRCNAQGTWE PSAPVCEKEC QAPPNILNGQ KEDRHMVRFD PGTSIKYSCN
PGYVLVGEES IQCTSEGVWT PPVPQCKVAA CEATGRQLLT KPQHQFVRPD VNSSCGEGYK
LSGSVYQECQ GTIPWFMEIR LCKEITCPPP PVIYNGAHTG SSLEDFPYGT TVTYTCNPGP
ERGVEFSLIG ESTIRCTSND QERGTWSGPA PLCKLSLLAV QCSHVHIANG YKISGKEAPY
FYNDTVTFKC YSGFTLKGSS QIRCKADNTW DPEIPVCEKE TCQHVRQSLQ ELPAGSRVEL
VNTSCQDGYQ LTGHAYQMCQ DAENGIWFKK IPLCKVIHCH PPPVIVNGKH TGMMAENFLY
GNEVSYECDQ GFYLLGEKKL QCRSDSKGHG SWSGPSPQCL RSPPVTRCPN PEVKHGYKLN
KTHSAYSHND IVYVDCNPGF IMNGSRVIRC HTDNTWVPGV PTCIKKAFIG CPPPPKTPNG
NHTGGNIARF SPGMSILYSC DQGYLLVGEA LLLCTHEGTW SQPAPHCKEV NCSSPADMDG
IQKGLEPRKM YQYGAVVTLE CEDGYMLEGS PQSQCQSDHQ WNPPLAVCRS RVEHHHHHH
Product Science Overview
CD21 is a 145 kDa N-glycosylated protein that contains a 951 amino acid extracellular domain with fifteen tandem short consensus repeats (SCRs) or sushi domains, a 28 amino acid transmembrane segment, and a 34 amino acid cytoplasmic tail . The extracellular domain of CD21 is responsible for binding to complement fragments such as C3d, C3dg, and iC3b, which are products of the complement cascade .
The primary function of CD21 is to enhance the immune response by facilitating the uptake and retention of immune complexes. It acts as a receptor for the Epstein-Barr virus (EBV), allowing the virus to infect B cells. Additionally, CD21 plays a pivotal role in B cell activation and the formation of immunological memory .
Recombinant human CD21 is produced using various expression systems, including HEK293 cells and Chinese Hamster Ovary (CHO) cells . The recombinant protein is typically expressed with a carboxy-terminal polyhistidine tag to facilitate purification. The molecular mass of the secreted recombinant human CD21 is approximately 106 kDa, but due to glycosylation, it appears as a 110-120 kDa band in SDS-PAGE under reducing conditions .
Recombinant CD21 is used in various research applications, including:
- Functional ELISA: To measure the binding ability of CD21 to its ligands, such as complement components and viral proteins .
- Immunophenotyping: To identify and study different subsets of B cells based on CD21 expression .
- Vaccine Development: As a target for developing vaccines against pathogens like EBV that utilize CD21 for entry into host cells .
