Introduction
The CD21 protein is found on mature B cells, follicular dendritic cells, and in lower amounts on immature thymocytes and T lymphocytes. During B cell development, CD21 appears after the pre-B cell stage, remains throughout peripheral B cell development, and disappears when the cells become plasma cells. When B cells are stimulated in vitro, CD21 expression is gradually lost. CD21 functions as a receptor for Complement components C3d, C3dg, and iC3b, for EBV, and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81, and Leu13 to form a large signal-transduction complex that is involved in B cell activation.
Formulation
The antibody is supplied as a 1 mg/ml solution in PBS after reconstitution.
Storage Procedures
The lyophilized antibody should be stored at 4 degrees Celsius. After reconstitution, the antibody should be used within one month. If it is not intended for use within one month, it should be aliquoted and stored at -20 degrees Celsius.
Solubility
Reconstitute the antibody with the appropriate volume of H2O. Mix gently by inverting the vial several times, then wash the sides of the vial with the reconstituted antibody. Wait 30-60 seconds before use.
Applications
This antibody is suitable for staining applications. The recommended concentration for staining is 10 µl of antibody per 1,000,000 cells.
Available Conjugates
This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody, use 5-10 µl of antibody per 1,000,000 cells.
Synonyms
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21 antigen, CR2, C3DR, CD21, SLEB9.
Purification Method
Protein-A.
Type
Mouse Anti Human Monoclonal.
Immunogen
Purified human B-Cells.