CD21 Antibody, Biotin
Description
Product Specs
Product Science Overview
CD21, also known as complement receptor 2 (CR2), is a 145 kDa transmembrane protein that plays a crucial role in the immune system. It is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells . CD21 is also known as the Epstein-Barr virus receptor, as it is used by the virus to infect B cells .
CD21 is characterized by multiple SUSHI domains and is involved in various immune responses. It acts as a receptor for the complement component C3d, which is a fragment of the complement protein C3 . This interaction is essential for the activation and regulation of B cells. CD21 also interacts with other proteins such as CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1) .
The mouse anti-human CD21 antibody is a monoclonal antibody that specifically binds to the CD21 protein on human cells. This antibody is often conjugated with biotin, a vitamin that allows for easy detection and purification of the antibody-antigen complex . The biotinylated mouse anti-human CD21 antibody is commonly used in various laboratory techniques, including flow cytometry, immunohistochemistry, and immunofluorescence .
- Flow Cytometry: The biotinylated mouse anti-human CD21 antibody is used to stain human peripheral blood lymphocytes, which can then be analyzed using flow cytometry . This technique helps in identifying and quantifying different cell populations based on their CD21 expression.
- Immunohistochemistry: This antibody can be used to stain acetone-fixed frozen sections, allowing researchers to visualize the distribution of CD21-expressing cells in tissue samples .
- Immunofluorescence: The biotinylated antibody can be used in combination with fluorescently labeled streptavidin to detect CD21 expression in various cell types .
