CD11b Antibody, FITC
Description
Product Specs
Integrins are proteins found on cell surfaces that help cells attach to each other and to other molecules. CD11b, also known as Mac-1 or CR3, is one such integrin found specifically on white blood cells like neutrophils and monocytes. It plays a crucial role in the immune system by helping these cells stick to blood vessel walls and engulf foreign particles coated with complement proteins. CD11b recognizes specific regions on these complement proteins, enabling efficient capture and removal of pathogens.
Product Science Overview
CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1 . This complex is involved in various cell-cell interactions by binding to its ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen . Mac-1 plays a significant role in the immune response, including phagocytosis, cell adhesion, and migration .
The Mouse Anti-Human CD11b antibody is a monoclonal antibody that specifically targets the CD11b antigen. It is commonly conjugated with FITC (Fluorescein Isothiocyanate), a fluorescent dye that allows for the visualization of the antibody-antigen interaction under a fluorescence microscope . The antibody is typically used in flow cytometry, immunofluorescence, and other immunoassays to detect and quantify CD11b expression on various cell types .
- Flow Cytometry: The FITC-conjugated CD11b antibody is widely used in flow cytometry to analyze the expression of CD11b on different cell populations .
- Immunofluorescence: This technique allows for the visualization of CD11b expression on cells and tissues using a fluorescence microscope .
- Immunohistochemistry: The antibody can be used to stain acetone-fixed frozen sections for the detection of CD11b .
