Introduction
Integrins are heterodimeric integral membrane proteins composed of an alpha and a beta chain. CD11b, an I-domain containing alpha integrin, partners with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin known as macrophage receptor 1 (MAC1). This integrin plays a critical role in the adhesion of neutrophils and monocytes to activated endothelium and the phagocytosis of complement-coated particles. This gene exhibits multiple transcript variants that encode various isoforms.
Formulation
Supplied at a concentration of 1 mg/ml in phosphate-buffered saline (PBS) following reconstitution.
Storage Procedures
The lyophilized product should be stored at 4 degrees Celsius. Upon reconstitution, it is recommended to use the antibody within one month. For long-term storage, aliquot the reconstituted antibody and store at -20 degrees Celsius.
Solubility
To reconstitute the antibody, add an appropriate volume of deionized water (H2O). Mix gently by inverting the vial several times and allow the solution to sit for 30-60 seconds before use. Ensure that the contents are fully dissolved and the solution is clear.
Applications
This antibody is suitable for blocking and staining applications. For staining, the recommended concentration is 10 microliters per 1,000,000 cells. The optimal titer for blocking LPS binding should be determined empirically by the investigator.
Available Conjugates
In addition to the unconjugated form, this antibody is also available in biotinylated and fluorescein isothiocyanate (FITC) conjugated forms. For staining with the biotin or FITC-conjugated antibody, use 5-10 microliters per 1,000,000 cells.
Synonyms
Integrin alpha-M, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b antigen, ITGAM, CR3A, MO1A, CD11B, MAC-1, MAC1A, MGC117044.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Immunogen
Purified human PBL Monocytes.
