CD11b Antibody, Biotin
CD11b, Mouse Anti-Human, Biotin
Cat. No.
BT28163
Source
Synonyms
Integrin alpha-M, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b antigen, ITGAM, CR3A, MO1A, CD11B, MAC-1, MAC1A, MGC117044.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Introduction
Integrins are heterodimeric integral membrane proteins composed of an alpha and a beta chain. CD11b, an alpha integrin with an I-domain, partners with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin known as macrophage receptor 1 (MAC1). This αMβ2 integrin plays a critical role in the adhesion of neutrophils and monocytes to activated endothelium and the phagocytosis of complement-coated particles. This gene exhibits multiple transcript variants that encode different isoforms.
Formulation
Supplied as a 1 mg/ml solution in PBS buffer. Reconstitute by adding deionized water.
Storage Procedures
Store the lyophilized antibody at 4°C. Following reconstitution, the antibody can be stored at 4°C for up to one month. For long-term storage, aliquot and freeze at -20°C.
Solubility
Reconstitute the antibody with deionized water. Gently mix the solution by inverting the vial several times. Allow the reconstituted antibody to stand for 30-60 seconds before use.
Applications
This antibody is suitable for blocking experiments and antibody staining procedures. For staining, it is recommended to use 10 µl of antibody per 10^6 cells. The optimal titer for blocking LPS binding should be determined empirically by the investigator.
Available Conjugates
In addition to the biotinylated format, this antibody is also offered unconjugated and conjugated to FITC. When using the biotin or FITC-conjugated antibody for staining, a concentration of 5-10 µl per 10^6 cells is recommended.
Synonyms
Integrin alpha-M, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b antigen, ITGAM, CR3A, MO1A, CD11B, MAC-1, MAC1A, MGC117044.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Clone
hCD11b.
Immunogen
Purified human PBL Monocytes.
Ig Subclass
Mouse IgG1.
Product Science Overview
Mouse Anti-Human CD11b Antibody
Applications
The biotin-conjugated Mouse Anti-Human CD11b Antibody has several applications in research and diagnostics:
- Flow Cytometry (FC): This antibody is commonly used in flow cytometry to identify and quantify CD11b-expressing cells .
- Immunohistochemistry (IHC): It is used in both frozen and acetone-fixed sections to detect CD11b expression .
- Immunoprecipitation (IP): The antibody can be used to precipitate CD11b from cell lysates .
- Immunofluorescence (IF): It is used in immunofluorescence microscopy to visualize CD11b-expressing cells .
Preparation and Storage
The antibody is purified by affinity chromatography and conjugated with biotin under optimal conditions . It is formulated in a phosphate-buffered solution with a pH of 7.2, containing 0.09% sodium azide as a preservative . The recommended storage conditions are between 2°C and 8°C, and the solution should not be frozen .
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