CD11b FITC Antibody

CD11b is a 170-kDa type 1 transmembrane glycoprotein . It is involved in various adhesive interactions of monocytes, macrophages, and granulocytes, as well as in mediating the uptake of complement-coated particles . This protein is essential for the immune response, particularly in processes such as phagocytosis, cell migration, and inflammation .

The Rat Anti-Mouse CD11b antibody is a monoclonal antibody that specifically targets the CD11b protein in mice. One of the commonly used clones for this purpose is clone 5C6 . This antibody is used in various research applications, including flow cytometry, immunoprecipitation, and immunofluorescence .

FITC (Fluorescein isothiocyanate) is a fluorescent dye that is commonly conjugated to antibodies for use in flow cytometry and other fluorescence-based assays . When conjugated to the Rat Anti-Mouse CD11b antibody, FITC allows for the visualization and quantification of CD11b expression on the surface of cells . The excitation and emission wavelengths for FITC are 495 nm and 519 nm, respectively .

The CD11b FITC, Rat Anti-Mouse antibody is widely used in immunological research to study the role of CD11b in various cellular processes. It is particularly useful in:

• Flow Cytometry: For the detection and quantification of CD11b expression on the surface of immune cells .
• Immunofluorescence: For the visualization of CD11b localization within cells .
• Immunoprecipitation: For the isolation and study of CD11b protein complexes .

Research using CD11b FITC, Rat Anti-Mouse antibody has provided significant insights into the mechanisms of immune cell adhesion, migration, and phagocytosis. It has also contributed to the understanding of inflammatory responses and the development of immune-related diseases .