C1QTNF1 Human
Complement C1q Tumor Necrosis Factor-Related Protein 1 Human Recombinant
Cat. No.
BT14277
Source
Escherichia Coli.
Synonyms
GIP, CTRP1, ZSIG37, FLJ90694, C1QTNF1, Complement C1q Tumor Necrosis Factor-Related Protein 1, G protein-coupled receptor-interacting protein.
Appearance
Purity
Greater than 95% as determined by SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
C1QTNF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 266 amino acids and having a molecular mass of 30.45 kDa. The protein contains an extra His tag at N-terminus. The C1QTNF1 amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q9BXJ1 amino acids 26–281.
The C1QTNF1 is purified by proprietary chromatographic techniques.
The C1QTNF1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
C1QTNF1, a newly discovered adipokine, plays a crucial role in understanding the biological functions and mechanisms of this family of secreted glycoproteins in both healthy and diseased states. It has been found to stimulate the production of aldosterone and is highly expressed in individuals with obesity and hypertension, suggesting a potential link between these conditions. Moreover, C1QTNF1 expression might be connected to low-grade chronic inflammation in adipose tissues.
Description
Recombinant human C1QTNF1, produced in E. coli, is a non-glycosylated polypeptide chain that consists of 266 amino acids. With a molecular weight of 30.45 kDa, it contains an additional His tag at the N-terminus. The amino acid sequence of this protein is identical to amino acids 26-281 of the UniProtKB/Swiss-Prot entry Q9BXJ1. Purification of C1QTNF1 is achieved through proprietary chromatographic methods.
Formulation
Human C1QTNF1 is lyophilized in a 50mM Acetate buffer with a pH of 4.
Solubility
To create a working stock solution of about 0.5mg/mL, add 0.1M Acetate buffer (pH 4) to the lyophilized pellet and allow it to completely dissolve. For use at a higher pH, dilute the solution with the appropriate buffer to a concentration of 10 µg/ml. It is important to note that the solubility of this antigen is limited at higher concentrations. The product is not sterile, so it must be filtered through a sterile filter before use in cell cultures.
Stability
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot the product to minimize repeated freezing and thawing cycles. Reconstituted protein remains stable at 4°C for a limited period, showing no significant changes after two weeks at this temperature.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
GIP, CTRP1, ZSIG37, FLJ90694, C1QTNF1, Complement C1q Tumor Necrosis Factor-Related Protein 1, G protein-coupled receptor-interacting protein.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS RVPHVQGEQQ EWEGTEELPS PPDHAERAEE QHEKYRPSQD QGLPASRCLR CCDPGTSMYP ATAVPQINIT ILKGEKGDRG DRGLQGKYGK TGSAGARGHT GPKGQKGSMG APGERCKSHY AAFSVGRKKP MHSNHYYQTV IFDTEFVNLY DHFNMFTGKF YCYVPGLYFF SLNVHTWNQK ETYLHIMKNE EEVVILFAQV GDRSIMQSQS LMLELREQDQ VWVRLYKGER ENAIFSEELD TYITFSGYLVKHATEP.
Product Science Overview
Introduction
Complement C1q Tumor Necrosis Factor-Related Protein 1 (C1QTNF1), also known as CTRP1, is a member of the C1q/TNF-related protein (CTRP) family. This family consists of secreted proteins that share structural similarities with the complement component C1q and tumor necrosis factor (TNF) superfamily. CTRPs are involved in various physiological processes, including metabolism, inflammation, and immune response.
Structure
C1QTNF1, like other CTRPs, is composed of several distinct domains:
- N-terminal signal peptide: This domain is responsible for directing the protein to the secretory pathway.
- Variable domain: This region shows variability among different CTRP family members.
- Collagenous domain: This domain contains Gly-X-Y repeats, which are characteristic of collagen proteins.
- C-terminal globular C1q (gC1q) domain: This domain is responsible for trimerization and is homologous to the globular domain of the complement protein C1q .
Function
C1QTNF1 plays a crucial role in regulating metabolism and inflammation. It has been shown to enhance insulin sensitivity and possess anti-inflammatory properties. The protein is involved in the following processes:
- Metabolism: C1QTNF1 enhances glucose uptake and fatty acid oxidation in skeletal muscle and adipose tissue. It also improves insulin sensitivity, making it a potential therapeutic target for metabolic disorders such as obesity and type 2 diabetes .
- Inflammation: C1QTNF1 exhibits anti-inflammatory effects by inhibiting the production of pro-inflammatory cytokines. This makes it a promising candidate for treating inflammatory diseases .
Receptors and Signaling Pathways
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