b NGF Human
Description
The NGF-b is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
β-NGF is a protein that belongs to the cysteine-knot family of growth factors, characterized by their stable dimeric structures . The molecular weight of human recombinant β-NGF is approximately 13.6 kDa . It is a potent neurotrophic factor that signals through its high-affinity receptor, tyrosine kinase A (trkA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR) .
β-NGF is essential for the survival and maintenance of sympathetic and sensory neurons . It regulates cell growth, promotes differentiation into neurons, and facilitates neuron migration . Additionally, β-NGF acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival . It also possesses pro-inflammatory and pro-fibrogenic properties .
The biological activity of β-NGF is mediated through its binding to the trkA receptor with high affinity and to the p75NTR with low affinity . This binding activates several intracellular signaling pathways, including the AKT and MAPK pathways, which are crucial for cell survival, differentiation, and proliferation .
Human recombinant β-NGF has several applications in research and medicine:
- Neuroscience Research: It is used to study the mechanisms of neuronal survival, differentiation, and growth.
- Stem Cell Research: β-NGF promotes the differentiation of bone marrow mesenchymal stem cells into neurons .
- Immunology: It plays a role in the regulation of the immune system and may function as an intracellular messenger to regulate the body’s response to stress .
- Therapeutic Potential: Due to its neurotrophic properties, β-NGF is being explored for potential therapeutic applications in neurodegenerative diseases and nerve injuries.
Human recombinant β-NGF is typically produced in expression systems such as HEK293 cells or E. coli . The protein is purified to a high degree, with purity levels exceeding 95% . It is available in various formulations, including animal component-free versions, to meet the needs of different research applications .
