Beta NGF Mouse
Beta Nerve Growth Factor Mouse Recombinant
Cat. No.
BT6970
Source
Escherichia Coli.
Synonyms
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Recombinant Mouse b-NGF produced in E.Coli is a noncovalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical chains of 120 amino acids each and having a molecular mass of 13,471 Dalton each.
The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology.
The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology.
Product Specs
Introduction
Beta-NGF, a protein with nerve growth stimulating properties, forms a complex involved in regulating the growth and differentiation of specific sensory and sympathetic neurons. Genetic mutations affecting this gene are linked to hereditary sensory and autonomic neuropathy, type 5 (HSAN5), while abnormal expression of this gene is associated with allergic rhinitis. Nerve Growth Factor holds the distinction of being the first discovered member of the neurotrophic factor family, which plays a crucial role in influencing the growth and differentiation of sensory and sympathetic neurons. NGF is a trimeric protein composed of alpha, beta, and gamma subunits, with the beta subunit being responsible for its growth-promoting activity. The production of NGF in astrocytes is stimulated by various cytokines, including IL1, TNF-alpha, PDGF, and TGF-beta.
Description
Recombinant Mouse b-NGF, expressed in E. coli, is a non-glycosylated homodimer linked by disulfide bonds. Each polypeptide chain consists of 120 amino acids, resulting in a molecular mass of 13,471 Daltons per chain. The purification of Recombinant Mouse-beta-NGF is achieved through advanced biological purification techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
The lyophilization of Recombinant Mouse b-NGF is carried out without the addition of any excipients.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Murine NGF-beta in sterile 18MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Mouse Beta-NGF, while stable at room temperature for a period of 3 weeks, should ideally be stored in a desiccated state at a temperature below -18°C. Following reconstitution, Murine NGF-Beta should be stored at 4°C for a duration of 2-7 days. For long-term storage, it is recommended to store it at -18°C. It is crucial to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 98%, as determined by the following methods: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of Mouse b-NGF was evaluated using a cell proliferation assay employing TF-1, a factor-dependent human erythroleukemic cell line. The half-maximal effective concentration (ED50) for this effect was determined to be 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg.
Synonyms
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Source
Escherichia Coli.
Amino Acid Sequence
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G.
Product Science Overview
Structure and Function
β-NGF is a protein composed of 118 amino acids and is highly conserved across different species . It is produced as a precursor protein, proNGF, which is then cleaved to form the mature, biologically active β-NGF . This neurotrophin binds to specific receptors on the surface of neurons, primarily the TrkA receptor and the p75 neurotrophin receptor (p75NTR), to exert its effects .
The primary functions of β-NGF include:
- Promoting Neuronal Survival: β-NGF supports the survival of neurons during development and in adulthood, particularly in the peripheral nervous system .
- Stimulating Neuronal Growth: It encourages the growth and branching of axons and dendrites, which are essential for the formation of neural networks .
- Facilitating Neuronal Differentiation: β-NGF promotes the differentiation of progenitor cells into mature neurons .
Production and Applications
Recombinant β-NGF is produced using genetic engineering techniques, where the gene encoding β-NGF is inserted into a host organism, such as bacteria or yeast, to produce the protein in large quantities . This recombinant form is used in various research and clinical applications.
Clinical Applications:
- Neurodegenerative Diseases: β-NGF has been investigated for its potential in treating neurodegenerative diseases such as Alzheimer’s disease, where it may help in maintaining the function of cholinergic neurons in the basal forebrain .
- Peripheral Neuropathies: It has shown promise in treating peripheral neuropathies by promoting the survival and regeneration of damaged sensory neurons .
- Wound Healing: β-NGF has been explored for its role in enhancing wound healing and tissue regeneration .
Research and Future Directions
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