ARL14 Human

ADP-Ribosylation Factor-Like 14 Human Recombinant
Cat. No.
BT13753
Source
Escherichia Coli.
Synonyms

ADP-ribosylation factor-like protein 14, ADP-ribosylation factor 7, ARL14, ARF7, FLJ22595.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARL14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-192 a.a.) and having a molecular mass of 23.8kDa (Molecular weight on SDS-PAGE will appear higher).
ARL14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The GTPase ARL14 (ADP-ribosylation factor-like 14) belongs to the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases and exhibits developmental regulation. ARL14 functions as a GTPase that recruits MYO1E to MHC class II-containing vesicles. This recruitment is mediated by the effector protein C11orf46/ARF7EP and plays a crucial role in controlling the movement of these vesicles along the actin cytoskeleton within dendritic cells. The GTPase ARL14/ARF7 governs MHC-II transport by recruiting the motor myosin 1E through the effector protein ARF7EP.
Description
Recombinant ARL14 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 212 amino acids (residues 1-192) and possesses a molecular mass of 23.8 kDa. It's important to note that the molecular weight on SDS-PAGE will appear higher. This protein is engineered with a 20 amino acid His-tag fused at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The ARL14 protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 40% glycerol, 200mM NaCl, and 1mM EDTA.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the ARL14 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms

ADP-ribosylation factor-like protein 14, ADP-ribosylation factor 7, ARL14, ARF7, FLJ22595.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSLGSKNPQ TKQAQVLLLG LDSAGKSTLL YKLKLAKDIT TIPTIGFNVE MIELERNLSL TVWDVGGQEK MRTVWGCYCE NTDGLVYVVD STDKQRLEES QRQFEHILKN EHIKNVPVVL LANKQDMPGA LTAEDITRMF KVKKLCSDRN WYVQPCCALT GEGLAQGFRK LTGFVKSHMK SRGDTLAFFK QN.

Product Science Overview

Gene and Protein Structure

The ARL14 gene is located on chromosome 3 and encodes a protein that is 192 amino acids in length . The protein is expressed in various tissues and is involved in the regulation of vesicle movement along the actin cytoskeleton in dendritic cells . This movement is facilitated by the recruitment of MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP .

Functional Role

ARL14 plays a crucial role in the immune system by controlling the movement of MHC class II-containing vesicles, which are essential for antigen presentation in dendritic cells . This function is vital for the immune response, as it helps in the activation of T-cells by presenting antigens on the cell surface .

Recombinant ARL14

Recombinant human ARL14 protein is produced using Escherichia coli expression systems and is purified to a high degree of purity (>90%) . This recombinant protein is used in various research applications, including mass spectrometry (MS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) . The recombinant protein retains the functional properties of the native protein, making it a valuable tool for studying the biological functions of ARL14 .

Clinical Significance

Recent studies have suggested that ARL14 may serve as a prognostic biomarker in non-small cell lung cancer (NSCLC) . The expression levels of ARL14 have been correlated with the progression of NSCLC, indicating its potential role in cancer biology . Further research is needed to fully understand the implications of ARL14 in cancer and other diseases.

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