ARL1 Human
Description
The ARL1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
ADP-Ribosylation Factor-Like 1 (ARL1) is a member of the ADP-ribosylation factor (ARF) family of GTP-binding proteins, which are part of the larger Ras superfamily. These proteins are ubiquitous in eukaryotic cells and play crucial roles in various cellular processes, including vesicular trafficking and actin cytoskeleton remodeling .
ARL1 is a small GTPase that cycles between an inactive GDP-bound form and an active GTP-bound form. This cycling is essential for its function in recruiting several effectors, such as golgins, arfaptins, and ARF-GEFs, to the trans-Golgi network. These interactions modulate various functions at the Golgi complex, including cell polarity, innate immunity, and protein secretion .
The protein consists of 181 amino acids and has several binding sites for GTP and magnesium ions, which are crucial for its activity. The conformational changes between the GDP-bound and GTP-bound states are characterized by alterations in the switch 1 and switch 2 regions, which bind tightly to the gamma-phosphate of GTP but poorly to GDP .
ARL1 plays a significant role in maintaining the structure and function of the Golgi apparatus. It is involved in the retrograde transport at the trans-Golgi network, which is essential for the proper sorting and trafficking of proteins within the cell. Additionally, ARL1 has been implicated in various cellular processes, including the maintenance of insulin secretion from pancreatic beta cells .
The human recombinant form of ARL1 is produced using recombinant DNA technology. This involves cloning the ARL1 gene into an expression vector, which is then introduced into a suitable host cell, such as Escherichia coli or yeast. The host cells are cultured under conditions that promote the expression of the recombinant protein, which is subsequently purified using various chromatographic techniques.
Recombinant ARL1 is used in various research applications to study its role in cellular processes and its interactions with other proteins. It is also used in structural studies to understand the conformational changes associated with its GTPase activity. Additionally, recombinant ARL1 can be used in drug discovery efforts to identify potential inhibitors or modulators of its activity.
