Ara h 9.0101
Description
Recombinant Ara h 9.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 9,959 Dalton.
Ara h 9.0101 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Lipid Transfer Proteins (LTPs) are a prevalent class of allergens found in various plant-based foods and pollens. These proteins are known to trigger immediate allergic reactions, including oral allergy syndrome. Notably, Ara h 9, an LTP found in peanuts, exhibits remarkable resistance to both enzymatic digestion and heat-induced denaturation, making it a potent allergen.
Recombinant Ara h 9.0101, synthesized in SF9 insect cells, is a glycosylated polypeptide with a molecular weight of 9,959 Daltons. The protein is engineered with a 6xHis tag at its N-terminus to facilitate purification, which is carried out using proprietary chromatographic methods.
Ara h 9.0101 is provided in a buffer solution consisting of 20mM HEPES at pH 7.9 and 6M Urea.
2. The protein's reactivity has been validated through immunodot assays using panels of positive and negative sera.
Product Science Overview
Allergen Ara h 9.0101 is a lipid transfer protein (LTP) derived from peanuts (Arachis hypogaea). LTPs are known for their role in plant defense mechanisms and are recognized as potent allergens in various plant foods and pollens. Ara h 9.0101 is particularly significant due to its association with severe allergic reactions, especially in peanut-allergic individuals from the Mediterranean region .
Recombinant Ara h 9.0101 is a glycosylated polypeptide with a calculated molecular mass of approximately 9,959 Daltons . It is expressed with a 6xHis tag at the N-terminus and purified using proprietary chromatographic techniques . The protein is highly resistant to digestion and heat denaturation, making it a stable allergen .
The recombinant form of Ara h 9.0101 is produced in Sf9 insect cells . The production process involves the expression of the protein in these cells, followed by purification through high-performance liquid chromatography (HPLC) gel filtration . The purified protein is then formulated in a phosphate-buffered saline solution, pH 7.4, and is sterile-filtered to ensure its purity and stability .
Ara h 9.0101 binds to IgE antibodies in human sera, making it a crucial component in the diagnosis and study of peanut allergies . It is used in immunodot tests with positive and negative sera panels to evaluate its allergenic potential . The protein’s ability to bind IgE antibodies highlights its role in triggering allergic reactions in sensitized individuals.
Recombinant Ara h 9.0101 is primarily used for laboratory research purposes. It is employed in studies aimed at understanding the immunological mechanisms underlying peanut allergies and in the development of diagnostic tools and therapeutic interventions . The protein is not intended for use as a drug, food additive, or household chemical .
