APOA1 Mouse

Apolipoprotein A-I Mouse Recombinant
Cat. No.
BT11265
Source
Escherichia Coli.
Synonyms
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, ProapoA-I.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

APOA1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (25-264a.a) and having a molecular mass of 30.3kDa.
APOA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
APOA1 (Apolipoprotein A-1) is a protein primarily responsible for lipid metabolism, serving as the major protein component of HDL in plasma. It facilitates the movement of cholesterol from tissues to the liver for excretion and acts as a cofactor for LCAT, an enzyme crucial for the formation of most plasma cholesteryl esters. APOA1 also plays a role in activating spermatozoa motility as part of the SPAP complex. The APOA1 gene, located on chromosome 11, is closely associated with two other apolipoprotein genes. Defects in this gene are linked to HDL deficiency, including conditions like Tangier disease and systemic non-neuropathic amyloidosis. Conversely, elevated levels of APOA1 are associated with the development of asthma and atopy.
Description
Recombinant Mouse APOA1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 263 amino acids (25-264 a.a.). With a molecular weight of 30.3 kDa, it comprises the APOA1 protein fused to a 23 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The APOA1 protein solution is provided at a concentration of 1 mg/ml and contains phosphate-buffered saline (pH 7.4), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the APOA1 Mouse Recombinant is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, ProapoA-I.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ.

Product Science Overview

Structure and Function

Apolipoprotein A-I is a 28 kDa glycoprotein that is predominantly produced by hepatocytes (about 75%) and epithelial cells of the small intestine (about 25%) . In mice, some ApoA-I is also produced by the most proximal part of the colon . The protein is composed of 243 amino acids and has a high affinity for phospholipids, which allows it to form HDL particles.

The primary function of ApoA-I is to promote cholesterol efflux from tissues by acting as a cofactor for the enzyme lecithin-cholesterol acyltransferase (LCAT). This enzyme is responsible for the esterification of cholesterol, which is a critical step in the formation of mature HDL particles . Additionally, ApoA-I has been shown to have anti-inflammatory and antioxidant properties, which contribute to its protective effects against cardiovascular diseases .

Recombinant ApoA-I

Recombinant ApoA-I is produced using various expression systems, with Escherichia coli being one of the most common hosts . The recombinant protein is typically purified to a high degree of purity (>90%) and is suitable for various applications, including SDS-PAGE and mass spectrometry . The recombinant form retains the functional properties of the native protein, making it a valuable tool for research and therapeutic purposes.

Applications and Research

Recombinant ApoA-I is widely used in research to study its role in lipid metabolism, cardiovascular diseases, and other related fields. It is also used in the development of synthetic HDL (sHDL) particles, which have shown promise in reducing atherosclerosis in animal models . These synthetic particles are designed to mimic the structure and function of natural HDL, providing a potential therapeutic approach for cardiovascular diseases.

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THE BIOTEK
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