APOA1 Human, His
Description
Product Specs
Product Science Overview
Apolipoprotein A-I (ApoA-I) is a crucial protein in human lipid metabolism, primarily known for its role in the formation of high-density lipoprotein (HDL) particles, often referred to as “good cholesterol.” The recombinant form of this protein, tagged with a polyhistidine (His) tag, is widely used in research and therapeutic applications.
ApoA-I is encoded by the APOA1 gene and is the major protein component of HDL particles . It plays a significant role in lipid metabolism by promoting the efflux of cholesterol from tissues to the liver for excretion, a process known as reverse cholesterol transport . This function is critical in preventing the buildup of cholesterol in the arteries, thereby reducing the risk of atherosclerosis and cardiovascular diseases .
The recombinant form of ApoA-I is produced using DNA sequences encoding the mature form of human ApoA-I. This sequence is expressed with a polyhistidine tag at the N-terminus, facilitating purification and detection . The recombinant protein consists of 263 amino acids and has a predicted molecular mass of approximately 30.7 kDa .
Recombinant ApoA-I is typically expressed in E. coli and purified using affinity chromatography techniques that exploit the His tag . The protein is lyophilized from a sterile PBS solution containing glycerol and other stabilizers to ensure its stability during storage and transport . The lyophilized protein can be reconstituted in a suitable buffer for experimental use.
Recombinant ApoA-I (His Tag) is used in various research applications, including studies on lipid metabolism, cardiovascular diseases, and cholesterol transport . It is also employed in the development of therapeutic strategies aimed at enhancing HDL function and reducing the risk of atherosclerosis .
