APOA1 Antibody
Description
Product Specs
Product Science Overview
Apolipoprotein A-I (ApoA-I) is a major protein component of high-density lipoprotein (HDL) in plasma. It plays a crucial role in the reverse transport of cholesterol from tissues to the liver for excretion. ApoA-I is a cofactor for the enzyme lecithin-cholesterol acyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters . Additionally, ApoA-I activates spermatozoa motility as part of the sperm-activating protein complex (SPAP) .
Mouse Anti Human Apolipoprotein A-I antibodies are monoclonal antibodies developed in mice that specifically target human ApoA-I. These antibodies are used in various scientific applications, including Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation . They are designed to react with both free human ApoA-I and HDL-bound ApoA-I, but do not cross-react with other apolipoproteins such as ApoE, ApoB, or albumin .
- Cholesterol Efflux: ApoA-I promotes the efflux of cholesterol from tissues to the liver, facilitating its excretion and thus playing a vital role in maintaining cholesterol homeostasis .
- LCAT Cofactor: As a cofactor for LCAT, ApoA-I is essential for the esterification of cholesterol, which is a critical step in the maturation of HDL particles .
- Sperm Motility: ApoA-I is involved in activating sperm motility, which is crucial for fertility .
The use of Mouse Anti Human Apolipoprotein A-I antibodies has significant implications in both research and clinical settings. These antibodies are employed to study the role of ApoA-I in various physiological and pathological processes, including cardiovascular diseases, lipid metabolism disorders, and fertility issues .
In research, these antibodies are used to quantify and analyze ApoA-I levels in different biological samples, such as plasma and tissue extracts. This helps in understanding the distribution and function of ApoA-I in health and disease . Clinically, they can be used in diagnostic assays to measure ApoA-I levels, which can provide valuable information about a patient’s lipid profile and cardiovascular risk .
