ALDOC Human, Active
Aldolase, Fructose-Bisphosphate C, Aldolase C, Fructose-Bisphosphate, Brain-Type Aldolase, EC 4.1.2.13, ALDC, Fructose-1,6-Biphosphate Triosephosphate Lyase, Fructose-Bisphosphate Aldolase C, Fructoaldolase C, Aldolase 3, ALDOC .
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
ALDOC Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 364 amino acids (1-364 a.a.) and having a molecular mass of 39.4kDa.
The ALDOC is purified by proprietary chromatographic techniques.
Product Specs
Aldolase, Fructose-Bisphosphate C, Aldolase C, Fructose-Bisphosphate, Brain-Type Aldolase, EC 4.1.2.13, ALDC, Fructose-1,6-Biphosphate Triosephosphate Lyase, Fructose-Bisphosphate Aldolase C, Fructoaldolase C, Aldolase 3, ALDOC .
MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY
Product Science Overview
Aldolase C is a member of the class I fructose-bisphosphate aldolase family. The human recombinant form of Aldolase C is expressed in Escherichia coli and is typically purified to a high degree of purity (>90%) using conventional chromatography techniques . The protein consists of 364 amino acids and has a molecular weight of approximately 39.4 kDa .
Aldolase C plays a crucial role in the fourth step of glycolysis, a metabolic pathway that breaks down glucose to produce energy. It is also involved in gluconeogenesis, the reverse pathway that generates glucose from non-carbohydrate precursors . The enzyme is specifically expressed in the hippocampus and Purkinje cells of the brain, indicating its importance in brain metabolism .
The specific activity of recombinant human Aldolase C is greater than 6 units/mg. One unit of the enzyme will convert 1.0 μmol of fructose-1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate per minute at pH 7.5 and 37°C . This high level of activity makes it suitable for various biochemical and physiological studies.
Recombinant human Aldolase C is used in research to study its role in metabolic pathways, particularly in glycolysis and gluconeogenesis. It is also utilized in structural biology to understand the enzyme’s mechanism of action and its interactions with other molecules. Additionally, it serves as a valuable tool in the development of therapeutic strategies for metabolic disorders and neurological diseases.
