ALDOC Human, His

Aldolase C Fructose-Bisphosphate Human Recombinant, His Tag
Cat. No.
BT26488
Source
Escherichia Coli.
Synonyms
Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ALDOC Human Recombinant fused to a 20 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 384 amino acids (1-364 a.a) and having a molecular mass of 41.6 kDa.
The ALDOC is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Aldolase C Fructose-Bisphosphate (ALDOC), a member of the class I fructose-bisphosphate aldolase family, is a glycolytic enzyme. It facilitates the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate into dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes, respectively. ALDOC expression is restricted to the brain's hippocampus and Purkinje cells.
Description
Recombinant human ALDOC, with a 20 amino acid His Tag fused at the N-terminal, is produced in E.Coli. This results in a single, non-glycosylated polypeptide chain consisting of 384 amino acids (1-364 a.a) and possessing a molecular weight of 41.6 kDa. Purification of ALDOC is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized through filtration.
Formulation
The ALDOC solution is provided at a concentration of 1mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing cycles should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 95.0%.
Synonyms
Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY.

Product Science Overview

Expression and Function

Aldolase C is specifically expressed in the hippocampus and Purkinje cells of the brain . It is a glycolytic enzyme that is essential for energy production in these brain regions. The enzyme’s activity is vital for maintaining the energy balance and proper functioning of neuronal cells .

Recombinant Aldolase C

The recombinant form of Aldolase C, tagged with a His (histidine) tag, is produced using Escherichia coli expression systems . This recombinant protein is typically purified to a high degree of purity (>95%) and is suitable for various applications such as SDS-PAGE and mass spectrometry (MS) . The His tag facilitates the purification process by allowing the protein to bind to nickel or cobalt ions, making it easier to isolate the protein from other cellular components .

Applications

Recombinant Aldolase C is used in various research applications, including:

  • Biochemical studies: Understanding the enzyme’s role in glycolysis and gluconeogenesis.
  • Structural biology: Investigating the three-dimensional structure of the enzyme.
  • Drug discovery: Screening for potential inhibitors or activators of the enzyme.
  • Neuroscience research: Studying the enzyme’s function in the brain and its role in neurological disorders.
Related Pathways and Diseases

Aldolase C is involved in key metabolic pathways such as glycolysis and gluconeogenesis . Dysregulation of these pathways can lead to various metabolic disorders. Additionally, mutations or alterations in the ALDOC gene have been associated with congenital disorders of glycosylation, which can affect multiple systems in the body .

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