ALDOB Human

Aldolase B Fructose-Bisphosphate Human Recombinant
Cat. No.
BT26257
Source
Escherichia Coli.
Synonyms
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDOB, ALDB, Aldolase B fructose-bisphosphate, ALDO2, aldolase 2, Aldolase B fructose-bisphosphatase.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ALDOB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 388 amino acids (1-364) and having a molecular mass of 42kDa.
ALDOB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Fructose-bisphosphate aldolase B (ALDOB) is a crucial enzyme involved in glycolysis, the process of breaking down glucose for energy. As a tetrameric enzyme, ALDOB consists of four subunits and plays a vital role in catalyzing the reversible conversion of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. This enzyme is primarily found in the kidney and small intestine of adults, where it works in conjunction with other aldolase isoenzymes, aldolase A or C. ALDOB's activity is regulated by hormones like insulin and glucagon, and its deficiency can lead to a metabolic disorder known as hereditary fructose intolerance.
Description
Recombinant human ALDOB, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein comprises 388 amino acids, with a molecular weight of 42kDa. It includes amino acids 1-364 of the ALDOB sequence and is fused to a 24 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The ALDOB solution is provided at a concentration of 1mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the ALDOB solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advisable. It's essential to avoid repeated freeze-thaw cycles to maintain the protein's integrity.
Purity
The purity of ALDOB is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDOB, ALDB, Aldolase B fructose-bisphosphate, ALDO2, aldolase 2, Aldolase B fructose-bisphosphatase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD
QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC
QAAKGQYVHT GSSGAASTQS LFTACYTY.

Product Science Overview

Gene and Structure

In humans, Aldolase B is encoded by the ALDOB gene located on chromosome 9 (9q31.1). The gene spans approximately 14,500 base pairs and contains 9 exons . The enzyme itself is a tetramer composed of identical 40-kilodalton subunits .

Function

Aldolase B catalyzes the reversible cleavage of fructose 1,6-bisphosphate (FBP) into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP). It also catalyzes the reversible cleavage of fructose 1-phosphate (F1P) into glyceraldehyde and dihydroxyacetone phosphate . This dual functionality is essential for the metabolism of fructose and the regulation of glycolytic and gluconeogenic pathways.

Expression and Isozymes

Aldolase B is predominantly expressed in the liver, kidney, and intestine. In contrast, Aldolase A is primarily found in muscle and erythrocytes, while Aldolase C is expressed in the brain . The expression pattern of these isozymes is tightly regulated and reflects their specialized roles in different tissues.

Clinical Significance

Mutations in the ALDOB gene can lead to hereditary fructose intolerance (HFI), a metabolic disorder characterized by the inability to properly metabolize fructose. This condition can result in severe hypoglycemia, abdominal pain, vomiting, and liver dysfunction upon ingestion of fructose .

Recombinant Aldolase B

Human recombinant Aldolase B is produced using recombinant DNA technology, which involves inserting the human ALDOB gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes. Recombinant Aldolase B retains the same structural and functional properties as the native enzyme, making it a valuable tool for studying fructose metabolism and related disorders.

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