ALDOC Antibody

Aldolase C, also known as fructose-bisphosphate aldolase C (ALDOC), is an enzyme encoded by the ALDOC gene located on chromosome 17 in humans . This enzyme is a member of the class I fructose-bisphosphate aldolase gene family and plays a crucial role in glycolysis and gluconeogenesis pathways . Aldolase C is predominantly expressed in the brain, specifically in the hippocampus and Purkinje cells of the cerebellum .

Aldolase C catalyzes the reversible aldol cleavage of fructose-1,6-bisphosphate and fructose 1-phosphate to produce dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively . This reaction is essential for the glycolytic pathway, which is a critical process for energy production in cells .

The mouse anti-human Aldolase C antibody is a monoclonal antibody derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ALDOC protein . This antibody is used in various research applications, including immunohistochemistry, western blotting, and enzyme-linked immunosorbent assays (ELISA), to detect and study the expression and function of Aldolase C in human tissues .

The mouse anti-human Aldolase C antibody has been instrumental in studying the role of Aldolase C in various physiological and pathological conditions. For instance, it has been used to investigate the enzyme’s involvement in neurodegenerative diseases, such as Alzheimer’s disease, where altered glycolytic pathways are observed . Additionally, this antibody helps in understanding the metabolic changes in cancer cells, as glycolysis is often upregulated in tumors .