ALDOC Mouse

Aldolase C Fructose-Bisphosphate Mouse Recombinant
Cat. No.
BT26553
Source
Escherichia Coli.
Synonyms
Aldolase 3, Brain-type aldolase, Scrapie-responsive protein 2, Zebrin II, Aldo3, Scrg2, Fructose-bisphosphate aldolase C, ALDOC.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

ALDOC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 387 amino acids (1-363 a.a) and having a molecular mass of 41.9kDa.
ALDOC is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Aldolase C Fructose-Bisphosphate (ALDOC) belongs to the class I fructose-bisphosphate aldolase family. ALDOC is a glycolytic enzyme which catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. ALDOC is expressed exclusively in the hippocampus and Purkinje cells of the brain.
Description
Recombinant ALDOC protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 387 amino acids (residues 1-363) with a molecular weight of 41.9 kDa. This protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The ALDOC protein is supplied in a solution at a concentration of 1 mg/ml, formulated in phosphate buffered saline (pH 7.4) containing 20% glycerol and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), store the protein solution at 4°C. For extended storage, freeze the solution at -20°C. To ensure protein stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the ALDOC protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Aldolase 3, Brain-type aldolase, Scrapie-responsive protein 2, Zebrin II, Aldo3, Scrg2, Fructose-bisphosphate aldolase C, ALDOC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPHSYP ALSAEQKKEL SDIALRIVTP GKGILAADES VGSMAKRLSQ IGVENTEENR RLYRQVLFSA DDRVKKCIGG VIFFHETLYQ KDDNGVPFVR TIQDKGILVG IKVDKGVVPL AGTDGETTTQ GLDGLLERCA QYKKDGADFA KWRCVLKISD RTPSALAILE NANVLARYAS ICQQNGIVPI VEPEILPDGD HDLKRCQYVT EKVLAAVYKA LSDHHVYLEG TLLKPNMVTP GHACPIKYSP EEIAMATVTA LRRTVPPAVP GVTFLSGGQS EEEASLNLNA INRCPLPRPW ALTFSYGRAL QASALNAWRG QRDNAGAATE EFIKRAEMNG LAAQGRYEGS GDGGAAAQSL YIANHAY

Product Science Overview

Definition and Classification

Aldolase C, also known as Fructose-Bisphosphate Aldolase C (ALDOC), is an enzyme that plays a crucial role in glycolysis and gluconeogenesis. It belongs to the class I fructose-bisphosphate aldolase family, which also includes aldolase A (ALDOA) and aldolase B (ALDOB) . This enzyme is specifically expressed in the brain, particularly in the hippocampus and Purkinje cells .

Biological Properties and Functions

Aldolase C catalyzes the reversible aldol cleavage of fructose-1,6-bisphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively . This reaction is a key step in the glycolytic pathway, which is essential for energy production in cells.

Recombinant Production

Recombinant Mouse Aldolase C is produced in Escherichia coli (E. coli) and is available as a full-length protein with a His-tag at the N-terminus . The recombinant protein is purified using proprietary chromatographic techniques to achieve a purity of over 95% . It is suitable for various applications, including SDS-PAGE and mass spectrometry (MS) .

Mode of Action

The enzyme’s mode of action involves the cleavage of fructose-1,6-bisphosphate into two three-carbon products, which are then further processed in the glycolytic pathway to produce ATP, the energy currency of the cell . This process is vital for maintaining cellular energy levels, especially in brain cells where Aldolase C is predominantly expressed.

Regulatory Mechanisms

The expression of Aldolase C is regulated at the transcriptional level and is influenced by various factors, including cellular energy status and metabolic demands . Additionally, post-translational modifications such as phosphorylation can affect the enzyme’s activity and stability.

Applications and Research

Recombinant Mouse Aldolase C is widely used in research to study its role in metabolism and its potential implications in neurological disorders. Its high purity and activity make it a valuable tool for biochemical assays and structural studies .

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