AHSG Mouse

Alpha-2-HS-Glycoprotein Mouse Recombinant
Cat. No.
BT18836
Source
Sf9, Baculovirus cells.
Synonyms
Alpha-2-HS-glycoprotein, Countertrypin, Fetuin-A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AHSG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 333 amino acids (19-345.a.) and having a molecular mass of 36.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
AHSG is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Alpha-2-HS-glycoprotein (AHSG), also known as fetuin-A, is a glycoprotein primarily produced by the liver. It plays various roles in the body, including regulating bone formation, influencing brain development, and participating in endocytosis. While its presence in developing tissues suggests its importance, the exact mechanisms of its functions are still being explored. AHSG binds to calcium and barium ions and exhibits properties related to endocytosis and opsonization.
Description
Recombinant AHSG protein, expressed in Sf9 insect cells using a baculovirus system, is a single polypeptide chain containing 333 amino acids (residues 19-345). This glycosylated protein has a molecular weight of 36.1 kDa, although it may appear between 40-57 kDa on SDS-PAGE due to glycosylation. The recombinant AHSG protein includes a 6-amino acid Histidine tag at the C-terminus to facilitate purification via proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AHSG protein solution is provided at a concentration of 0.25 mg/ml in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the AHSG protein solution should be kept at 4°C. For extended storage, it is recommended to store the solution at -20°C. Adding a carrier protein, such as HSA or BSA at a final concentration of 0.1%, is advised for long-term storage. To preserve protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the AHSG protein is greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
Alpha-2-HS-glycoprotein, Countertrypin, Fetuin-A.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
APQGTGLGFR ELACDDPEAE QVALLAVDYL NNHLLQGFKQ VLNQIDKVKV WSRRPFGVVY EMEVDTLETT CHALDPTPLA NCSVRQLTEH AVEGDCDFHI LKQDGQFRVM HTQCHSTPDS AEDVRKLCPR CPLLTPFNDT NVVHTVNTAL AAFNTQNNGT YFKLVEISRA QNVPLPVSTL VEFVIAATDC TAKEVTDPAK CNLLAEKQHG FCKANLMHNL GGEEVSVACK LFQTQPQPAN ANAVGPVPTA NAALPADPPA SVVVGPVVVP RGLSDHRTYH DLRHAFSPVA SVESASGETL HSPKVGQPGA AGPVSPMCPG RIRHFKIHHH HHH.

Product Science Overview

Properties

AHSG is a major plasma protein and a member of the cystatin superfamily of protease inhibitors . It has a high affinity for calcium and barium ions, which suggests its role in bone metabolism . The protein is more abundant in fetal blood than in adult blood, indicating its significant role during development .

Functions

The primary functions of AHSG include promoting endocytosis, possessing opsonic properties, and influencing the mineral phase of bone . It is also involved in the regulation of bone mineralization, negative regulation of biomineral tissue development, and skeletal system development . Additionally, AHSG plays a role in the negative regulation of insulin receptor signaling pathway and positive regulation of phagocytosis .

Modes of Action

AHSG exerts its effects through various mechanisms. It promotes endocytosis and has opsonic properties, which means it can enhance the immune system’s ability to target and eliminate pathogens . Its high affinity for calcium ions allows it to influence bone mineralization and development . AHSG is also involved in the regulation of inflammatory responses and the negative regulation of kinase activity .

Regulatory Mechanisms

The expression of AHSG is regulated at multiple levels, including transcriptional and post-transcriptional mechanisms . It is synthesized by hepatocytes and adipocytes and is present in the serum . The protein undergoes post-translational modifications, including cleavage from a proprotein encoded from a single mRNA . These regulatory mechanisms ensure the proper functioning and availability of AHSG in the body.

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THE BIOTEK
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