AHSG Human
Alpha-2-HS-Glycoprotein Human Recombinant
Cat. No.
BT18686
Source
Escherichia Coli.
Synonyms
PRO2743, A2HS, AHS, FETUA, HSGA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
AHSG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 372 amino acids (19-367 a.a) and having a molecular mass of 39.7kDa (Molecular size on SDS-PAGE will appear higher).
AHSG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
AHSG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Alpha-2-HS-glycoprotein (AHSG), also known as fetuin-A, is a glycoprotein primarily synthesized by the liver. As a member of the fetuin family of plasma binding proteins, AHSG plays diverse roles in various biological processes, including endocytosis, brain development, and bone tissue formation. Its presence in the developing cerebral cortex and bone marrow suggests a potential contribution to tissue development, although its precise function in these contexts remains to be fully elucidated. Notably, AHSG exhibits properties such as promoting endocytosis, exhibiting opsonic activity, influencing bone mineralization, and demonstrating an affinity for calcium and barium ions.
Description
Recombinant human AHSG, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 372 amino acids (residues 19-367). This protein variant has a molecular weight of 39.7 kDa, although it may appear larger on SDS-PAGE due to the presence of a 23 amino acid His-tag at the N-terminus. The purification process involves proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The AHSG protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the recombinant AHSG is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
PRO2743, A2HS, AHS, FETUA, HSGA, Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSAPHGPGL IYRQPNCDDP ETEEAALVAI DYINQNLPWG YKHTLNQIDE VKVWPQQPSG ELFEIEIDTL ETTCHVLDPT PVARCSVRQL KEHAVEGDCD FQLLKLDGKF SVVYAKCDSS PDSAEDVRKV CQDCPLLAPL NDTRVVHAAK AALAAFNAQN NGSNFQLEEI SRAQLVPLPP STYVEFTVSG TDCVAKEATE AAKCNLLAEK QYGFCKATLS EKLGGAEVAV TCTVFQTQPV TSQPQPEGAN EAVPTPVVDP DAPPSPPLGA PGLPPAGSPP DSHVLLAAPP GHQLHRAHYD LRHTFMGVVS LGSPSGEVSH PRKTRTVVQP SVGAAAGPVV PPCPGRIRHF KV.
Product Science Overview
Structure and Composition
Functions
Alpha-2-HS-Glycoprotein is involved in several critical functions:
- Bone Mineralization: AHSG plays a role in the regulation of bone mineralization, which is essential for skeletal development .
- Immune Response: It is involved in the immune response, particularly in the regulation of inflammation .
- Endocytosis and Brain Development: AHSG is implicated in endocytosis and brain development, contributing to the formation of bone tissue .
- Acute-Phase Reactant: Clinically, AHSG is considered a negative acute-phase reactant, with its levels decreasing during inflammation .
Clinical Significance
AHSG levels have clinical implications in various conditions:
- Cancer: Concentrations of AHSG are reduced in cancer patients .
- Gestational Diabetes: AHSG levels are positively correlated with gestational diabetes .
- Neonatal Skeletal Development: There is a negative correlation between AHSG levels and neonatal skeletal development .
- Endometriosis: High concentrations of AHSG are found in the peritoneal fluids of patients with endometriosis .
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