ACE2 (18-740) Human, Biotin
HEK293 Cells
ACE-2 Protein is >90% pure as determined SDS-PAGE.
Description
The HEK293 derived ACE2 Human recombinant biotinylated protein contains the amino acids Gln 18-Ser 740 fused to His-AVI tag at C-terminal having a predicted Mw of 87.2 kDa ( migrates 95-125 kDa under reducing conditions on sds-page due to glycosilation). ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.
Product Specs
Angiotensin-converting enzyme 2 (ACE2) is an enzyme found on the surface of cells in various organs, including the intestines, arteries, lungs, heart, and kidneys. It serves as an entry point for SARS coronaviruses, including SARS-CoV-2. The spike (S) glycoprotein of coronaviruses plays a crucial role in viral infection by binding to host cell receptors, such as ACE2, and facilitating the fusion of viral and cellular membranes. The S protein has two main domains: the N-terminal domain and the C-terminal domain. One or both of these domains can function as receptor-binding domains. SARS-CoV and MERS-CoV both utilize their C-domain to bind to receptors. ACE2 is a transmembrane protein with an extracellular N-terminal domain containing the catalytic site and an intracellular C-terminal tail. It acts as a mono-carboxypeptidase, which means it can cleave specific peptide bonds. ACE2 is involved in converting angiotensin I to angiotensin 1-9 and angiotensin II to angiotensin 1-7, playing a role in regulating blood pressure and other physiological processes.
This recombinant ACE2 protein is produced in HEK293 cells and corresponds to amino acids Gln 18 to Ser 740 of the human ACE2 protein. It is biotinylated and has a His-AVI tag at the C-terminus. The predicted molecular weight is 87.2 kDa, but it migrates at 95-125 kDa on SDS-PAGE under reducing conditions due to glycosylation. This protein binds to the receptor-binding domain of the SARS-CoV-2 spike protein.
The ACE2 human recombinant protein solution is supplied in 50mM Tris buffer (pH 7.5) containing 150mM NaCl and 20% glycerol.
The ACE-2 Human Recombinant Protein is shipped with ice packs. Upon arrival, it should be stored at -20°C. For long-term storage, adding 0.1% albumin is highly recommended.
The purity of the ACE-2 protein is determined to be greater than 90% by SDS-PAGE analysis.
The biological activity of ACE2 was assessed using a functional ELISA, specifically by measuring its binding ability. The immobilized recombinant human ACE2 protein demonstrates binding affinity to the SARS-CoV-2 Spike protein Receptor Binding Domain at a concentration of 2 µg/ml.
HEK293 Cells
Purified by Protein-G chromatographic technique.
Product Science Overview
Angiotensin Converting Enzyme 2 (ACE2) is a crucial enzyme in the renin-angiotensin system (RAS), which regulates blood pressure, fluid and electrolyte balance, and systemic vascular resistance. The specific form of ACE2 discussed here is a biotinylated human recombinant version, encompassing amino acids 18 to 740.
The recombinant ACE2 protein is produced in HEK293 cells, a human embryonic kidney cell line, which ensures proper folding and post-translational modifications. This version of ACE2 includes a His-AVI tag at the C-terminal, facilitating purification and detection . The biotinylation allows for easy attachment to streptavidin-coated surfaces, which is useful in various biochemical assays.
ACE2 plays a pivotal role in converting angiotensin I (Ang I) to angiotensin 1-9 and angiotensin II (Ang II) to angiotensin 1-7. Unlike its homolog ACE, which converts Ang I to Ang II, ACE2 generates peptides that counterbalance the vasoconstrictive and proliferative effects of Ang II. Angiotensin 1-7, for instance, acts as a vasodilator and has anti-proliferative properties .
ACE2 gained significant attention as the functional receptor for the SARS-CoV-2 virus, which causes COVID-19. The virus’s spike protein binds to ACE2, facilitating viral entry into host cells . This interaction has made ACE2 a target for therapeutic interventions aimed at preventing or treating COVID-19.
The biotinylated recombinant ACE2 protein is widely used in research to study its enzymatic activity, interaction with the SARS-CoV-2 spike protein, and potential therapeutic applications. It is also employed in drug screening assays to identify inhibitors that could block the virus-ACE2 interaction .
