ACE2 (19-740) Human
CHO Cells
Protein is >95% pure as determined SDS-PAGE.
Description
The CHO derived ACE2 Human recombinant protein contains the extracellular domain amino acids 19-740 fused to Fc tag at C-terminal. ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.
Product Specs
Angiotensin-converting enzyme 2 (ACE2) is an enzyme found on the surface of cells in various organs, including the intestines, arteries, lungs, heart, and kidneys. It serves as an entry point for SARS coronaviruses, including SARS-CoV-2. The spike (S) glycoprotein of the coronavirus, a class I viral fusion antigen on the virion's outer envelope, plays a crucial role in viral infection. It does this by binding to host cell receptors and facilitating the fusion of viral and cellular membranes. The S1 subunit of the spike protein has two main domains, the N-terminal domain and the C-terminal domain, one or both of which function as receptor-binding domains. SARS-CoV and MERS-CoV both utilize their C-domains to bind to their receptors. ACE2 is a transmembrane protein with an extracellular N-terminal domain containing the catalytic site and an intracellular C-terminal tail. ACE2 has a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing a HEXXH zinc-binding domain. It acts as a mono-carboxypeptidase, breaking down Ang I to produce the nonapeptide Ang 1-9 and Ang II to create the heptapeptide Ang 1-7.
This recombinant human ACE2 protein, derived from CHO cells, consists of the extracellular domain (amino acids 19-740) fused to an Fc tag at the C-terminus. It binds to the receptor-binding domain of the SARS-CoV-2 spike protein.
This human ACE2 protein solution is supplied in 50mM Tris-HCl (pH 7.5) and 90mM glycine.
This recombinant human ACE2 protein is shipped on ice packs. Upon arrival, it should be stored at -20°C.
The protein purity is greater than 95% as determined by SDS-PAGE.
ACE2 activity was assessed via its binding ability using a functional ELISA. The immobilized recombinant human ACE2 protein exhibits binding to the SARS-CoV-2 spike protein receptor-binding domain at a concentration of 2 µg/ml.
CHO Cells
Purified by Protein-G chromatographic technique.
Product Science Overview
Angiotensin Converting Enzyme 2 (ACE2) is a crucial enzyme in the renin-angiotensin system (RAS), which plays a significant role in regulating cardiovascular and renal functions. The recombinant form of this enzyme, particularly the segment spanning amino acids 19-740, has garnered attention for its potential therapeutic applications and its role in various physiological processes.
The human recombinant ACE2 (19-740 a.a.) is typically produced using mammalian expression systems, such as HEK293 cells . This recombinant protein includes a His-tag for purification purposes and is often supplied in a liquid form, stored in phosphate-buffered saline (PBS) at pH 7.4 . The amino acid sequence of this recombinant protein corresponds to the human ACE2 sequence from glutamine at position 18 to serine at position 740 .
ACE2 is a monocarboxypeptidase that metabolizes several peptides, including the conversion of angiotensin II (Ang II) to angiotensin 1-7 (Ang 1-7) . Ang 1-7 exerts vasodilatory and antiproliferative effects by acting through its receptor, Mas . This action counterbalances the classical pathway of the RAS, which involves the ACE-Ang II-Ang II type-1 receptor axis . The ACE2/Ang 1-7/Mas receptor axis plays a protective role in cardiovascular health by mitigating the adverse effects of Ang II, such as vasoconstriction and proliferation .
The recombinant human ACE2 has shown promise in preclinical models and clinical studies for its potential therapeutic benefits in conditions like heart failure, hypertension, and acute lung injury . By increasing the Ang 1-7/Ang II ratio, recombinant ACE2 helps to restore the balance in the RAS, leading to improved cardiovascular outcomes . Additionally, ACE2 has been identified as a functional receptor for the SARS coronavirus, highlighting its importance in infectious disease research .
Recombinant ACE2 is widely used in research to study its role in various physiological and pathological processes. It is employed in assays such as Western Blot (WB) and ELISA to investigate its expression and function . The protein’s ability to bind to the SARS-CoV-2 spike receptor binding domain has also made it a valuable tool in COVID-19 research .
