ACE2 (18-615) Human
CHO Cells
Protein is >95% pure as determined SDS-PAGE.
Description
The CHO derived ACE2 Human recombinant protein contains the extracellular domain amino acids 18- 615 fused to Fc tag at C-terminal and has a molecular weight of ~130 kDa. ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.
Product Specs
Angiotensin-converting enzyme 2 (ACE2) is an enzyme found on the surface of cells in various organs, including the intestines, arteries, lungs, heart, and kidneys. ACE2 acts as an entry point for SARS coronaviruses, including SARS-CoV-2, the virus responsible for COVID-19. The spike (S) glycoprotein of the coronavirus is a key protein that enables the virus to infect cells. It does this by binding to ACE2 on the host cell surface. The S protein has two main domains: the N-terminal domain and the C-terminal domain. Both of these domains can bind to ACE2. SARS-CoV and MERS-CoV use their C-domain to attach to ACE2. ACE2 itself is a transmembrane protein, meaning it spans the cell membrane. It has an extracellular part, a transmembrane part, and an intracellular part. The extracellular part contains the catalytic site, which is where the enzyme's activity takes place. ACE2 acts as a mono-carboxypeptidase, meaning it cleaves off the terminal amino acid from a protein chain. In the case of ACE2, it cleaves angiotensin I to produce angiotensin 1-9 and angiotensin II to produce angiotensin 1-7.
This recombinant ACE2 protein is derived from Chinese hamster ovary (CHO) cells. It contains the extracellular domain of human ACE2 (amino acids 18-615) fused to an Fc tag at the C-terminus. This protein has a molecular weight of approximately 130 kDa and binds to the receptor binding domain of the SARS-CoV-2 spike protein.
The ACE2 Human protein solution is supplied in 50mM Tris-HCl, pH 7.5, and 90mM glycine.
The ACE-2 Human Recombinant Protein is shipped on ice packs. Upon arrival, it should be stored at -20°C.
The protein purity is greater than 95% as determined by SDS-PAGE.
The ACE2 activity was assessed using a functional ELISA to measure its binding ability. The immobilized Recombinant Human ACE2 protein demonstrates binding to the SARS CoV2 Spike protein Receptor Binding Domain at a concentration of 2 µg/ml.
CHO Cells
Product Science Overview
Angiotensin Converting Enzyme 2 (ACE2) is a crucial enzyme in the renin-angiotensin system (RAS), which plays a significant role in regulating cardiovascular functions and maintaining homeostasis. The recombinant form of ACE2, particularly the segment spanning amino acids 18-615, has garnered considerable attention due to its involvement in various physiological and pathological processes, including its role as a receptor for coronaviruses such as SARS-CoV and SARS-CoV-2.
ACE2 is a type I transmembrane protein that belongs to the zinc metalloprotease family. It is composed of an extracellular N-terminal domain, which contains the catalytic site, a transmembrane domain, and an intracellular C-terminal tail. The recombinant form of ACE2 (18-615 a.a.) includes the extracellular domain fused to an Fc tag at the C-terminal, resulting in a protein with a molecular weight of approximately 130 kDa .
The primary function of ACE2 is to act as a mono-carboxypeptidase, converting angiotensin II (Ang II) into angiotensin-(1-7) (Ang-(1-7)), which has vasodilatory and anti-inflammatory properties. This conversion counterbalances the effects of Ang II, which include vasoconstriction, inflammation, and fibrosis .
ACE2 gained widespread recognition during the COVID-19 pandemic as the cellular receptor for the SARS-CoV-2 virus. The spike (S) glycoprotein of the virus binds to the ACE2 receptor on the host cell surface, facilitating viral entry and infection . This interaction is critical for the virus’s ability to infect human cells and has made ACE2 a focal point in research aimed at understanding and combating COVID-19.
Given its role in the RAS and as a receptor for SARS-CoV-2, ACE2 has become a target for therapeutic interventions. Strategies to modulate ACE2 activity or block its interaction with the viral spike protein are being explored to develop treatments for COVID-19 and other related diseases . Additionally, recombinant ACE2 proteins are being investigated for their potential to neutralize the virus and mitigate its effects on the body .
The recombinant form of ACE2 (18-615 a.a.) is produced using Chinese Hamster Ovary (CHO) cells and is supplied as a sterile filtered solution. It is used extensively in laboratory research to study its binding interactions with the SARS-CoV-2 spike protein and to explore its therapeutic potential . The protein is highly pure, with a purity level exceeding 95% as determined by SDS-PAGE, and its biological activity is confirmed through functional assays .
