Y.Enterocolitica (O:9) YopN
Description
Y.Enterocolitica (O:9) YopN is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Yersinia enterocolitica is a Gram-negative, rod-shaped bacterium belonging to the Enterobacteriaceae family. It is known for causing yersiniosis, an infection that affects both humans and animals, including cattle, deer, pigs, and birds . The bacterium is motile at temperatures between 22-29°C but becomes non-motile at normal human body temperature .
Yersinia enterocolitica is classified into various serogroups based on its O (lipopolysaccharide or LPS) surface antigen. There are more than 57 O serogroups, with serogroups O:3, O:5,27, O:8, and O:9 being the most frequently isolated from human samples . In Europe, serogroup O:3 is predominant, followed by O:9, while in the United States, serogroup O:8 is more common .
The YopN protein is one of the virulence factors produced by Yersinia enterocolitica. It plays a crucial role in the bacterium’s ability to cause disease by regulating the secretion of other virulence proteins. YopN is part of the Yersinia outer proteins (Yops) family, which are essential for the bacterium’s pathogenicity.
The recombinant Yersinia enterocolitica (O:9) YopN is produced in Escherichia coli (E. coli) and is a non-glycosylated polypeptide chain with a calculated molecular mass of 34,296 Daltons . It is expressed with a 10xHis tag at the N-terminus and purified using proprietary chromatographic techniques .
The recombinant YopN protein is supplied as a sterile filtered clear solution in a formulation containing 20mM HEPES buffer (pH 7.6), 250mM NaCl, and 20% glycerol . It has a purity greater than 80.0% as determined by SDS-PAGE . The protein should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods to avoid multiple freeze-thaw cycles .
