Y.Enterocolitica (O:9) YopH
Description
Y.Enterocolitica (O:9) YopH is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Yersinia enterocolitica is classified into various serotypes based on the structure of its lipopolysaccharides (LPS). One of the significant serotypes is O:9, which is known for its pathogenicity and ability to cause disease in humans. This serotype has been implicated in several outbreaks of yersiniosis, a disease characterized by symptoms such as diarrhea, fever, and abdominal pain.
The YopH protein is a virulence factor produced by Yersinia enterocolitica. It is a tyrosine phosphatase enzyme that plays a crucial role in the bacterium’s ability to evade the host immune system. By dephosphorylating key signaling molecules in host cells, YopH disrupts normal cellular functions, thereby aiding in the bacterium’s survival and proliferation within the host.
Recombinant YopH refers to the YopH protein that has been produced using recombinant DNA technology. This involves inserting the gene encoding YopH into a suitable expression system, such as E. coli, to produce the protein in large quantities. The recombinant YopH protein is typically tagged with a histidine (His) tag to facilitate its purification using chromatographic techniques .
Recombinant YopH has several applications in research and diagnostics:
- Immunological Studies: It is used to study the immune response to Yersinia infections. By binding to human antibodies of the IgG, IgM, and IgA types, recombinant YopH can help in understanding the host-pathogen interactions.
- Diagnostic Tests: Recombinant YopH is used in immunodot tests to differentiate between positive and negative sera panels, aiding in the diagnosis of yersiniosis.
- Vaccine Development: Research on recombinant YopH can contribute to the development of vaccines against Yersinia infections by identifying potential antigenic targets.
Recombinant YopH is typically supplied in a sterile filtered solution containing HEPES buffer, NaCl, and glycerol. It is recommended to store the protein at -20°C for long-term stability, although it can be kept at 4°C if used within 2-4 weeks. Multiple freeze-thaw cycles should be avoided to maintain the protein’s integrity .
