Y.Enterocolitica (O:9) LcrV
Description
Y.Enterocolitica (O:9) LcrV is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Yersinia enterocolitica is a Gram-negative, bacillus-shaped bacterium belonging to the Enterobacteriaceae family . It is motile at temperatures between 22-29°C but becomes non-motile at normal human body temperature . This bacterium is known to cause yersiniosis, a zoonotic disease that affects humans and various animals, including cattle, deer, pigs, and birds .
Yersinia enterocolitica is classified into six biogroups based on phenotypic characteristics and more than 57 “O” serogroups based on their O (lipopolysaccharide or LPS) surface antigen . Among these, serogroups O:3, O:5,27, O:8, and O:9 are most frequently isolated from human samples . In many European countries, serogroup O:3 is predominant, followed by O:9, while O:8 is more common in the United States .
The low calcium response V antigen (LcrV) is a crucial component of the bacterial translocation apparatus, necessary for the secretion of Yersinia outer proteins (Yops) involved in host cell infection . The LcrV protein plays a significant role in the virulence of Yersinia species, making it a target for vaccine development .
The recombinant Yersinia enterocolitica (O:9) LcrV protein is produced in Escherichia coli (E. coli) expression systems . This recombinant protein is a non-glycosylated polypeptide chain with a calculated molecular mass of approximately 38,668 Daltons . It is expressed with a 10xHis tag at the N-terminus and purified using proprietary chromatographic techniques .
The recombinant LcrV protein is supplied as a sterile filtered clear solution in a formulation containing 20mM HEPES buffer (pH 7.6), 250mM NaCl, and 20% glycerol . It has a purity greater than 80% as determined by SDS-PAGE . The protein should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods to avoid multiple freeze-thaw cycles .
