VEGF Mouse (121 a.a.), Yeast
Saccharomyces cerevisiae
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Vascular Endothelial Growth Factor (121 a.a.) Mouse Recombinant produced in yeast is a disulfide-linked homodimer consisting of 2x121 amino acid polypeptide chains, having a molecular mass of approximately 20.7kDa each.
VEGF (121 a.a.) is purified by proprietary chromatographic techniques.
Product Specs
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Saccharomyces cerevisiae
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R.
Product Science Overview
Vascular Endothelial Growth Factor (VEGF) is a critical signaling protein involved in both vasculogenesis (the formation of new blood vessels during embryonic development) and angiogenesis (the growth of blood vessels from pre-existing vasculature). VEGF is essential for the formation of the circulatory system and plays a pivotal role in both physiological and pathological processes.
VEGF belongs to the platelet-derived growth factor (PDGF) family and is characterized by the presence of eight conserved cysteine residues and a cysteine-knot structure . The VEGF family consists of several isoforms, including VEGF121, VEGF165, VEGF189, and VEGF206, which are generated through alternative splicing of the VEGF gene. VEGF121 is one of the shortest isoforms, consisting of 121 amino acids .
The mouse recombinant VEGF121 produced in yeast is a disulfide-linked homodimer, consisting of two 121 amino acid polypeptide chains. Each chain has a molecular mass of approximately 20.7 kDa . This recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and activity.
VEGF121 is a potent mediator of angiogenesis and vasculogenesis. It binds to VEGF receptors (VEGFR-1 and VEGFR-2) on the surface of endothelial cells, triggering a cascade of signaling pathways that promote endothelial cell proliferation, migration, and new blood vessel formation . This activity is crucial for tissue growth and repair, as well as for the progression of certain diseases, such as cancer, where angiogenesis is a key factor in tumor growth and metastasis.
Recombinant VEGF121 is widely used in research to study angiogenesis and related processes. It has therapeutic potential in treating conditions characterized by poor blood supply, such as ischemic heart disease and peripheral artery disease. Additionally, VEGF121 has been investigated for its role in mitigating the effects of pre-eclampsia, a serious hypertensive disorder of pregnancy .
