VEGF Mouse, Yeast

Vascular endothelial growth factor (VEGF) is a key signaling protein involved in the formation of new blood vessels, both through angiogenesis (from existing vessels) and vasculogenesis (de novo formation). While its effects are most well-studied on vascular endothelial cells (lining blood vessels), VEGF also impacts other cell types like immune cells (monocytes/macrophages), neurons, cancer cells, and kidney epithelial cells. VEGF contributes to increased blood vessel permeability, stimulates angiogenesis and vasculogenesis, promotes endothelial cell growth and migration, and inhibits cell death (apoptosis). In laboratory settings, VEGF has been shown to stimulate endothelial cell division (mitogenesis) and movement (migration). Additionally, VEGF acts as a vasodilator, widening blood vessels, and enhances the permeability of small blood vessels. It was initially referred to as vascular permeability factor due to this property. Notably, elevated VEGF levels are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Furthermore, genetic mutations in VEGF have been associated with both proliferative and nonproliferative diabetic retinopathy, a condition affecting the eyes.

This recombinant Vascular Endothelial Growth Factor protein is produced in yeast and designed to mimic the native mouse protein. It exists as a disulfide-linked homodimer, meaning it comprises two identical polypeptide chains linked by a disulfide bond. Each chain consists of 165 amino acids, resulting in a molecular mass of approximately 40.0kDa per chain. The total molecular weight of the dimer is therefore around 80.0 kDa. The protein is purified using proprietary chromatographic techniques to ensure its quality and purity.

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein is provided as a lyophilized powder. It was originally formulated in a concentrated solution of phosphate-buffered saline (PBS) at a pH of 7.4. The solution was sterile-filtered through a 0.2 µm filter before freeze-drying.

To reconstitute the lyophilized Vascular Endothelial Growth Factor, it is recommended to dissolve it in sterile water with a resistance of 18 megaohm-centimeters (18 MΩ·cm). The minimum concentration for reconstitution is 100 micrograms per milliliter (100 µg/ml). This solution can then be further diluted to desired concentrations using other aqueous solutions.

The lyophilized VEGF protein remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated (dry) environment below -18°C. After reconstitution, the Vascular Endothelial Growth Factor solution should be stored at 4°C and used within 2-7 days. For extended storage, it can be aliquoted and stored below -18°C. Avoid repeated freeze-thaw cycles to maintain protein stability.

The purity of this protein is greater than 97.0%, as determined by the following methods:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.

The biological activity of this protein is measured by its ability to stimulate the proliferation of human umbilical vein endothelial cells (HUVEC). The ED50, which represents the concentration of VEGF required to achieve half-maximal cell proliferation, is in the range of 1.0-5.0 nanograms per milliliter (ng/ml).

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Saccharomyces cerevisiae

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.