TSLP Human

Thymic Stromal Lymphopoietin Human Recombinant
Cat. No.
BT23506
Source
Escherichia Coli.
Synonyms
Thymic Stromal Lymphopoietin, TSLP.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.
The TSLP is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a role in immune responses, particularly in allergic inflammation. It signals through a receptor complex composed of the thymic stromal lymphopoietin receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα). TSLP primarily affects myeloid cells, such as monocytes and dendritic cells, promoting the release of chemokines that attract T cells and enhancing dendritic cell maturation. TSLP is expressed in various tissues, including the heart, liver, and prostate. Its biological activities are similar to IL-7, and it activates similar signaling pathways, including STAT3 and STAT5 phosphorylation, although it does not utilize Janus kinases (JAKs). One of the key roles of TSLP is in allergic inflammation. It promotes the production of pro-inflammatory cytokines, such as interleukin-4 (IL-4), IL-5, IL-13, and TNF-alpha, by T cells while suppressing the production of anti-inflammatory cytokines, such as IL-10 and IFN-gamma. This imbalance in cytokine production contributes to the development and exacerbation of allergic responses.
Description
Recombinant human TSLP, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 132 amino acids. It has a molecular weight of 15 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein is provided as a lyophilized powder, which has been freeze-dried from a concentrated solution containing 130mM NaCl, 20mM sodium phosphate, at pH 7.4.
Solubility
To reconstitute the lyophilized TSLP, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized TSLP remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product desiccated at a temperature below -18°C. After reconstitution, TSLP can be stored at 4°C for 2 to 7 days. For extended storage, it is advisable to store the reconstituted solution at a temperature below -18°C, preferably with the addition of a carrier protein (0.1% HSA or BSA) to enhance stability. It's essential to prevent repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of this protein is greater than 97.0%, as determined by two methods:
(a) Analysis using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC).
(b) Analysis using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of this protein is determined by its ability to induce the proliferation of mouse BaF3 cells co-transfected with human Interleukin-7 receptor alpha and human TSLP Receptor. The ED50, which represents the concentration of TSLP required to achieve half-maximal proliferation, is less than 0.3 ng/ml.
Synonyms
Thymic Stromal Lymphopoietin, TSLP.
Source
Escherichia Coli.
Amino Acid Sequence
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ.

Product Science Overview

Structure and Function

TSLP is an interleukin-7 (IL-7)-like cytokine that interacts with a receptor complex composed of the TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα) . This interaction activates downstream signaling pathways that are essential for immune responses . TSLP is known for its role in promoting T-helper type 2 (TH2) immune responses, which are involved in allergic reactions and asthma .

Biological Roles

TSLP has multiple functions and is involved in various physiological and pathological conditions:

  1. Immune Cell Proliferation and Development: TSLP was initially appreciated for its role in the proliferation and development of immune cells .
  2. Type 2 Immune Responses: It plays a pivotal role in type 2 immune responses, which are crucial in allergic diseases and asthma .
  3. Autoimmune Diseases and Cancer: TSLP is also involved in autoimmune diseases and certain cancers .
Recombinant TSLP

Human recombinant TSLP is a laboratory-produced version of the naturally occurring cytokine. It is used in research and therapeutic applications to study its effects and potential treatments for diseases involving the immune system. Recombinant TSLP contains specific mutations to eliminate potential cleavage sites, ensuring its stability and functionality .

Clinical Implications

Understanding the role of TSLP in immune responses has significant clinical implications. Targeting TSLP can be a therapeutic strategy to disrupt the inflammatory cascade in allergic diseases and asthma, potentially alleviating symptoms associated with these conditions . Moreover, its involvement in autoimmune diseases and cancer opens avenues for research into novel treatments .

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