TSLP Mouse
Sf9, Baculovirus cells.
Thymic Stromal Lymphopoietin, TSLP.
Greater than 90.0% as determined by SDS-PAGE.
Description
TSLP Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 130 amino acids (20-140aa) and having a molecular mass of 15.0kDa.
TSLP is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a crucial role in the immune system. It signals through a receptor complex composed of the TSLP receptor and the interleukin-7 receptor alpha chain. TSLP primarily affects myeloid cells, stimulating the release of chemokines that attract T cells and promoting the maturation of dendritic cells. Primarily found in the heart, liver, and prostate, TSLP exhibits biological similarities to IL-7, binding to the same receptor complex. Both cytokines activate STAT3 and STAT5 signaling pathways, although TSLP utilizes different kinases for activation. TSLP's role in allergic inflammation is significant, as it prompts monocytes to release chemokines like TARC and MDC, attracting T cells, and activates dendritic cells to prime naive T cells for producing pro-allergic cytokines like IL-4, IL-5, IL-13, and TNF-alpha while suppressing IL-10 and IFN-gamma production.
Recombinant Mouse TSLP, produced in a Baculovirus expression system, is a single, glycosylated polypeptide chain comprising 130 amino acids (residues 20-140). With a molecular weight of 15.0 kDa, the protein includes a 6-amino acid His-tag fused to its C-terminus. Purification is achieved through proprietary chromatographic techniques.
The TSLP solution is supplied at a concentration of 0.5 mg/ml in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
The purity of the TSLP protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Thymic Stromal Lymphopoietin, TSLP.
Sf9, Baculovirus cells.
ADPYNFSNCN FTSITKIYCN IIFHDLTGDL KGAKFEQIED CESKPACLLK IEYYTLNPIP
GCPSLPDKTF ARRTREALND HCPGYPETER NDGTQEMAQE VQNICLNQTS QILRLWYSFM QSPEHHHHHH
Product Science Overview
Thymic Stromal Lymphopoietin (TSLP) is a cytokine that plays a crucial role in the immune system. It was originally identified from the conditioned medium of a mouse thymic stromal cell line, where it promoted the development of B cells . TSLP is known for its involvement in various physiological and pathological processes, primarily those related to the immune system .
TSLP is an interleukin (IL)-2-like cytokine and shares a common ancestor with IL-7 . It exerts its biological effects by binding to a high-affinity heteromeric complex composed of the TSLP receptor chain and IL-7Rα . This binding activates antigen-presenting cells, which in turn help mature the T cell population in the body . TSLP is primarily expressed by epithelial cells, but it can also be produced by dendritic cells, mast cells, and other immune cells .
There are two main isoforms of TSLP in human tissues: the short form (sfTSLP) and the long form (lfTSLP). The short form is expressed in steady-state conditions and plays a homeostatic role, while the long form is upregulated in inflammatory conditions . In mice, TSLP is expressed in various tissues, including the thymus, lungs, and intestines .
TSLP is involved in a variety of immune responses. It plays a pivotal role in type 2 immune responses and is implicated in allergic diseases such as atopic dermatitis, bronchial asthma, and eosinophilic esophagitis . Emerging evidence also indicates that TSLP is involved in chronic inflammatory disorders like chronic obstructive pulmonary disease (COPD) and celiac disease, as well as autoimmune diseases such as psoriasis and rheumatoid arthritis .
Given its broad role in immune responses, TSLP has become a target for therapeutic interventions. Tezepelumab, a first-in-class human monoclonal antibody, binds to TSLP and inhibits its interaction with the TSLP receptor complex . Clinical trials have shown that Tezepelumab is effective and safe as an add-on therapy for patients with severe uncontrolled asthma .
