TSHB Human
TSHB
Description
Product Specs
Thyroid-stimulating hormone beta (TSHB) is produced by thyrotrope cells in the anterior pituitary gland. It plays a crucial role in regulating the thyroid gland's production of thyroid hormones, namely triiodothyronine (T3) and thyroxine (T4). The hypothalamus secretes thyrotropin-releasing hormone (TRH), which stimulates TSHB production and release. Conversely, somatostatin, also produced by the hypothalamus, inhibits TSHB release. A feedback loop exists where blood levels of T3 and T4 influence TSHB production. Low T3 and T4 levels stimulate TSHB production, while high levels suppress it.
TSHB
Product Science Overview
The production of TSHB is controlled by Thyrotropin-Releasing Hormone (TRH), which is synthesized in the hypothalamus and transported to the anterior pituitary gland. TRH increases the production and release of TSHB. Conversely, somatostatin, also produced by the hypothalamus, inhibits the release of TSHB .
The levels of T3 and T4 in the blood create a regulatory negative feedback loop. When the levels of T3 and T4 are low, the production of TSHB is increased, and when the levels are high, the production is decreased .
Recombinant TSHB is produced using Escherichia coli (E. coli) as the host organism. The recombinant form is a single, non-glycosylated polypeptide chain containing 141 amino acids and has a molecular mass of 15.9 kDa. It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .
The recombinant TSHB is typically formulated as a sterile filtered clear solution containing 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea, and 10% glycerol. It is recommended to store the solution at 4°C if it will be used within 2-4 weeks, or frozen at -20°C for longer periods. For long-term storage, adding a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA) is advised to prevent freeze-thaw cycles .
Recombinant TSHB is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical. Researchers utilize recombinant TSHB to study thyroid function, hormone regulation, and related disorders such as hypothyroidism and Hashimoto’s thyroiditis .
The TSHB gene encodes the beta subunit of thyroid-stimulating hormone. This gene is part of a family of glycoprotein hormones that include chorionic gonadotropin (CG), luteinizing hormone (LH), and follicle-stimulating hormone (FSH). These hormones are dimers consisting of alpha and beta subunits that are associated non-covalently. The alpha subunits are identical across these hormones, but the beta chains are unique and confer biological specificity .
Mutations in the TSHB gene can lead to congenital central and secondary hypothyroidism and other thyroid-related disorders. The gene is located on chromosome 1 and has multiple transcript variants due to alternative splicing .
