scIC2

Single Chain Cardiac Troponin I-C 2nd Generation Recombinant (scIC2) is a specialized protein used in cardiovascular research and diagnostics. This recombinant protein is engineered to mimic the natural cardiac troponin complex, which plays a crucial role in muscle contraction regulation, particularly in the heart.

The scIC2 is a single, non-glycosylated polypeptide chain produced in Escherichia coli (E. coli). It consists of amino acids 28-110 of cardiac troponin I (cTnI) linked to cardiac troponin C (cTnC). The molecular weight of this recombinant protein is approximately 30 kDa . The protein solution is formulated with 20mM Tris-HCl, 250mM NaCl, and 50% glycerol at pH 7.5 .

The production of scIC2 involves recombinant DNA technology, where the gene encoding the desired protein is inserted into E. coli bacteria. These bacteria then express the protein, which is subsequently purified using proprietary chromatographic techniques to achieve a purity greater than 91% as determined by SDS-PAGE .

scIC2 is primarily used in immunoassays and western blotting for cardiovascular research. It serves as a calibrator and control material for cardiac troponin I immunoassays, providing superior calibration and control compared to traditional methods . This makes it an invaluable tool for researchers studying heart diseases and developing diagnostic tests.

The stability of scIC2 is maintained at 10°C for up to 7 days, but for long-term storage, it should be kept below -18°C to prevent freeze-thaw cycles, which can degrade the protein .